Avsunviroidae 190 claims

6 entries

Peach latent mosaic viroid105 claims · 44 papers Avocado sunblotch viroid53 claims · 19 papers Eggplant latent viroid12 claims · 2 papers Pelamoviroid malleusmalialso Apple hammerhead viroid10 claims · 6 papers Chrysanthemum chlorotic mottle viroid9 claims · 5 papers Avsunviroidae1 claim · 1 paper

190 claims

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Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: DCL1ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL1e0
Outcome: ELVd accumulatione0
Direction: enhancee0

Not reportedComparator

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: DCL2ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL2e0
Outcome: ELVd accumulatione0
Direction: enhancee0

Not reportedComparator

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: DCL3ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL3e0
Outcome: ELVd accumulatione0
Direction: enhancee0

Not reportedComparator

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: DCL4ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL4e0
Outcome: ELVd accumulatione0
Direction: enhancee0

Not reportedComparator

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectauthor interpretationoriginal resultno effectmeasured

Claim · model-written summary

RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: RDR6ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Outcome: ELVd accumulatione0
Direction: little impacte0
Qualifier: seemse0

Not reportedComparator

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Mechanismobservedoriginal resultmeasured

Claim · model-written summary

DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL2e4
Outcome: generating 22 nt ELVd siRNAe4

Verbatim

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

e4
“Through small RNA profiling, we unravel that DCL2 plays an essential role in generating 22 nt ELVd siRNA”
✓ located verbatim in the source · abstract, characters 731–835

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Mechanismobservedoriginal resultmeasured

Claim · model-written summary

DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL3e5
Outcome: biogenesis of 24 nt chloroplastic viroid siRNA (cvd-siRNA)e5
Qualifier: in the absence of DCL2e5

Verbatim

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

e5
“DCL3 is functionally redundant to DCL2 and targets ELVd for biogenesis of 24 nt chloroplastic viroid siRNA (cvd-siRNA) in the absence of DCL2”
✓ located verbatim in the source · abstract, characters 837–978

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: ELVd infectione3
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Gene: DCL1e3
Outcome: DCL1-processed miRNAse3
Direction: reducese3
Qualifier: in both wild-type and RNAi plantse3

Not reportedComparator

Verbatim

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

e3
“ELVd infection reduces DCL1-processed miRNAs in both wild-type and RNAi plants”
✓ located verbatim in the source · abstract, characters 1108–1186

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Intervention effectauthor interpretationoriginal resultmeasured

Claim · model-written summary

RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: RDR6ie0
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Outcome: cvd-siRNA abundancee5
Qualifier: at the late stage of ELVd infectione5

Not reportedComparatorDirection

Verbatim

e0
“DCL1i , DCL2i , DCL3i , and DCL4i enhance, whilst RDR6i seems to have little impact on, ELVd accumulation”
✓ located verbatim in the source · abstract, characters 624–729

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

e5
“affects cvd-siRNA abundance at the late stage of ELVd infection”
✓ located verbatim in the source · abstract, characters 1353–1416

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.
Observation Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Chloroplastic small RNAs change dynamically during eggplant latent viroid infection.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Interactor: eggplant latent viroidEggplant latent viroide2pathogen
Outcome: dynamic changes of csRNAse7
Qualifier: during ELVd infectione7

Verbatim

e1
“transgenic Nicotiana benthamiana RNAi lines RDR6i and DCLsi”
✓ located verbatim in the source · abstract, characters 464–523

e2
“utilizing eggplant latent viroid (ELVd), a chloroplast replicating viroid”
✓ located verbatim in the source · abstract, characters 375–448

e7
“The dynamic changes of csRNAs during ELVd infection”
✓ located verbatim in the source · abstract, characters 1558–1609

Title

RNA silencing response in chloroplast-replicating viroid siRNA biogenesis in plants

Pengcheng Zhang; Xinlian Zhang; Atef M. Mohamed; et al. Phytopathology Research 2025. doi:10.1186/s42483-025-00351-3

Other claims from this paper (13)

Intervention effect DCL1 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL2 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL3 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect DCL4 knockdown enhanced eggplant latent viroid accumulation in Nicotiana benthamiana.
Intervention effect RDR6 knockdown seems to have little impact on eggplant latent viroid accumulation in Nicotiana benthamiana.
Mechanism DCL2 plays an essential role in generating 22 nt eggplant latent viroid siRNA.
Mechanism DCL3 is functionally redundant with DCL2 and drives biogenesis of 24 nt chloroplastic viroid siRNA in the absence of DCL2.
Mechanism DCL4 accounts for the less abundant 21 nt chloroplastic viroid siRNA production.
Mechanism DCL1 does not contribute to chloroplastic viroid siRNA biogenesis.
Intervention effect Eggplant latent viroid infection reduced DCL1-processed miRNAs in both wild-type and RNAi Nicotiana benthamiana plants.
Intervention effect RDR6 knockdown has no influence on the 21–24 nt size profile of chloroplastic viroid siRNA.
Intervention effect RDR6 affects chloroplastic viroid siRNA abundance at the late stage of eggplant latent viroid infection.
Mechanism A different processing might produce chloroplastic small RNAs (csRNAs) in chloroplasts.