By interacting organism › Viruses › Pamosaviridae

Observationobservedoriginal resultmeasured

Claim · model-written summary

Preliminary results: fractions from monocots infected with Panicum mosaic virus and satellite panicum mosaic virus contained ribonuclease activity, siRNAs and Argonaute-domain proteins.

Taxon: monocotsLiliopsidae3
Interactor: Panicum mosaic virusPanicum mosaic viruse3pathogen
Second interactor: satellite panicum mosaic virusPanicum mosaic satellite viruse3pathogen
Outcome: ribonuclease activitye4
Qualifier: Preliminarye4

Verbatim

e3
“monocots were infected with Panicum mosaic virus (PMV) and satellite panicum mosaic virus (SPMV)”
✓ located verbatim in the source · abstract, characters 1045–1141

e4
“Preliminary studies show that fractions contained ribonuclease activity as well as siRNAs and proteins containing an Argonaute domain”
✓ located verbatim in the source · abstract, characters 1212–1345

Title

Anti-viral RNAi and its suppression in plants.

Jessica J. Ciomperlik OakTrust (Texas A&M University Libraries) 2010

Other claims from this paper (7)

Observation Fractions from Nicotiana benthamiana infected with TBSV and TRV contained a virus-specific ribonuclease activity co-eluting with siRNAs.
Intervention effect Ribonuclease activity in Nicotiana benthamiana fractions was sensitive to inhibition by EDTA.
Intervention effect Divalent metal cations enhanced the ribonuclease activity in Nicotiana benthamiana fractions.
Observation The ribonuclease activity co-purified with proteins containing an Argonaute-family domain.
Intervention effect The hordeivirus protein ?b enhanced expression of the foreign protein GFP early in infection.
Intervention effect The potyvirus protein HcPro and tombusvirus protein P19 enhanced and extended protein production later in infection.
Intervention effect Cointroduced suppressors of RNAi can extend the production of a foreign protein.

Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

The dosage of SPMV CP affects symptom severity, supporting its role as a pathogenicity factor.

Taxon: host plantse1
Interactor: Satellite panicum mosaic virusPanicum mosaic satellite viruse1pathogen
Gene: SPMV CPe2
Outcome: symptom severitye2

Not reportedDirection

Verbatim

e1
“Satellite panicum mosaic virus (SPMV) depends on its helper Panicum mosaic virus (PMV) for replication and movement in host plants”
✓ located verbatim in the source · abstract, characters 0–130

e2
“the dosage effect of SPMV CP on symptom severity supports its function as a pathogenicity factor”
✓ located verbatim in the source · abstract, characters 582–678

Title

Molecular biology study of satellite panicum mosaic virus capsid protein

Qi Dong OakTrust (Texas A&M University Libraries) 2007

Other claims from this paper (13)

Intervention effect SPMV CP shows compensatory effects on virus mutants defective in systemic movement in host plants.
Observation SPMV CP is involved in cell-to-cell movement of SPMV RNA in host plants.
Observation SPMV CP is dispensable for symptom exacerbation in host plants.
Observation SPMV CP in the cytosol exists exclusively as virions.
Observation The N-terminal arginine-rich motif of SPMV CP is required for in vitro binding of SPMV and PMV genomic RNAs.
Intervention effect Mutations of the N-ARM region impair all known functions of SPMV CP.
Intervention effect Manipulation of the C-terminus of SPMV CP gives the same phenotypes as alterations in the N-ARM.
Intervention effect Manipulation of the C-terminus of SPMV CP does not affect its RNA binding activity.
Observation Virions are not required for SPMV CP to facilitate local and systemic movement.
Observation Virions are not required for SPMV CP to inhibit the accumulation of SPMV DIs.
Mechanism SPMV CP and RNA may form alternative complexes for facilitating movement and inhibiting DI accumulation.
Observation SPMV CP localizes to the nucleolus.
Observation SPMV CP interacts with PMV CP in the form of virions.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

SPMV CP shows compensatory effects on virus mutants defective in systemic movement in host plants.

Taxon: host plantse1
Treatment: virus mutants defective in systemic movemente2
Interactor: Satellite panicum mosaic virusPanicum mosaic satellite viruse1pathogen
Gene: SPMV CPe2
Outcome: systemic movemente2

Not reportedComparatorDirection

Verbatim

e1
“Satellite panicum mosaic virus (SPMV) depends on its helper Panicum mosaic virus (PMV) for replication and movement in host plants”
✓ located verbatim in the source · abstract, characters 0–130

e2
“Biological assays also demonstrate compensatory effects of SPMV CP on virus mutants defective in systemic movement”
✓ located verbatim in the source · abstract, characters 680–794

Title

Molecular biology study of satellite panicum mosaic virus capsid protein

Qi Dong OakTrust (Texas A&M University Libraries) 2007

Other claims from this paper (13)

Association The dosage of SPMV CP affects symptom severity, supporting its role as a pathogenicity factor.
Observation SPMV CP is involved in cell-to-cell movement of SPMV RNA in host plants.
Observation SPMV CP is dispensable for symptom exacerbation in host plants.
Observation SPMV CP in the cytosol exists exclusively as virions.
Observation The N-terminal arginine-rich motif of SPMV CP is required for in vitro binding of SPMV and PMV genomic RNAs.
Intervention effect Mutations of the N-ARM region impair all known functions of SPMV CP.
Intervention effect Manipulation of the C-terminus of SPMV CP gives the same phenotypes as alterations in the N-ARM.
Intervention effect Manipulation of the C-terminus of SPMV CP does not affect its RNA binding activity.
Observation Virions are not required for SPMV CP to facilitate local and systemic movement.
Observation Virions are not required for SPMV CP to inhibit the accumulation of SPMV DIs.
Mechanism SPMV CP and RNA may form alternative complexes for facilitating movement and inhibiting DI accumulation.
Observation SPMV CP localizes to the nucleolus.
Observation SPMV CP interacts with PMV CP in the form of virions.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The SPMV capsid protein induced severe chlorosis on proso millet plants.

Taxon: Panicum miliaceumPanicum miliaceume1
Material: cv. Sunup or Red Turghaie1
Interactor: Satellite panicum mosaic virusPanicum mosaic satellite viruse0pathogen
Gene: SPMV CPe1
Outcome: chlorosise1
Qualifier: severee1

Verbatim

e0
“Satellite panicum mosaic virus (SPMV), an 824-nucleotide, positive-sense, single-stranded RNA virus”
✓ located verbatim in the source · abstract, characters 0–99

e1
“identifies additional biological activities of the SPMV CP, including the induction of severe chlorosis on proso millet plants (Panicum miliaceum cv. Sunup or Red Turghai)”
✓ located verbatim in the source · abstract, characters 443–614

Title

Genetic Identification of Multiple Biological Roles Associated with the Capsid Protein of Satellite Panicum Mosaic Virus

Wenping Qiu; Karen-Beth G. Scholthof Molecular Plant-Microbe Interactions 2001. doi:10.1094/mpmi.2001.14.1.21

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Deletion mutagenesis mapped the chlorosis-inducing domain to amino acids 50 to 157 of the SPMV capsid protein.

Taxon: Panicum miliaceumPanicum miliaceume1
Interactor: Satellite panicum mosaic virusPanicum mosaic satellite viruse0pathogen
Gene: SPMV CPe2
Outcome: chlorosis-inducing domaine2
Value: amino acids 50 to 157e2

Verbatim

e2
“Initial deletion mutagenesis experiments mapped the chlorosis-inducing domain to amino acids 50 to 157 on the C-terminal portion of the SPMV CP”
✓ located verbatim in the source · abstract, characters 616–759

e1
“identifies additional biological activities of the SPMV CP, including the induction of severe chlorosis on proso millet plants (Panicum miliaceum cv. Sunup or Red Turghai)”
✓ located verbatim in the source · abstract, characters 443–614

e0
“Satellite panicum mosaic virus (SPMV), an 824-nucleotide, positive-sense, single-stranded RNA virus”
✓ located verbatim in the source · abstract, characters 0–99

Title

Genetic Identification of Multiple Biological Roles Associated with the Capsid Protein of Satellite Panicum Mosaic Virus

Wenping Qiu; Karen-Beth G. Scholthof Molecular Plant-Microbe Interactions 2001. doi:10.1094/mpmi.2001.14.1.21

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Amino acids 124 to 135 formed a critical domain for chlorosis induction and virion formation.

Taxon: Panicum miliaceumPanicum miliaceume1
Interactor: Satellite panicum mosaic virusPanicum mosaic satellite viruse0pathogen
Gene: SPMV CPe1
Outcome: chlorosis induction and virion formatione3
Value: amino acids 124 to 135e3

Verbatim

e3
“amino acids 124 to 135 comprised a critical domain associated with chlorosis induction and virion formation”
✓ located verbatim in the source · abstract, characters 797–904

e1
“identifies additional biological activities of the SPMV CP, including the induction of severe chlorosis on proso millet plants (Panicum miliaceum cv. Sunup or Red Turghai)”
✓ located verbatim in the source · abstract, characters 443–614

e0
“Satellite panicum mosaic virus (SPMV), an 824-nucleotide, positive-sense, single-stranded RNA virus”
✓ located verbatim in the source · abstract, characters 0–99

Title

Genetic Identification of Multiple Biological Roles Associated with the Capsid Protein of Satellite Panicum Mosaic Virus

Wenping Qiu; Karen-Beth G. Scholthof Molecular Plant-Microbe Interactions 2001. doi:10.1094/mpmi.2001.14.1.21

Other claims from this paper (6)

Pamosaviridae 6 claims

6 claims