Claim · model-written summary
Authentication of commercial wine grape material by PCR was unsuccessful due to insufficient residual DNA.
- Taxon
- grapee2
- Outcome
- authentication of grape material by PCRe2
- Qualifier
- insufficient amount of residual DNAe2
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“In case of commercial wines authentication of grape material by PCR using wine as a biological material was unsuccessfully due to insufficient amount of residual DNA in samples analyzed”
Title
DNA Fingerprinting Used for Testing Some Romanian Wine Varieties
Monica Hârța; Doru Pamfil; Rodica Pop; et al. http://journals.usamvcluj.ro/index.php/horticulture/article/download/7041/6302/ 2011. doi:10.15835/buasvmcn-hort:7041
Other claims from this paper (5)
- Method / resource Residual grape DNA could be detected in wine up to 18 months after fermentation and was suitable for DNA fingerprinting.
- Method / resource Experimental 12-month-old wine samples were more amplified than the 18-month-old ones.
- Method / resource Among the microsatellites tested, VVS2 and VVMD7 gave satisfactory results.
- Method / resource The VVS2 marker generated the shortest PCR amplicons, of 119-138 bp.
- Observation The VVS2 marker amplified all of the grapevine cultivars analyzed in the wine samples.