Claim · model-written summary
The authors recommend using the modified cleared leaf method to measure stomatal size and density in the middle of leaves and branchlets.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“the use of our modified cleared leaf method to acquire accurate stomatal size and whole-leaf stomatal density measurements from the middle portion of a leaf located at the middle portion of a branchlet”
t0“conifer”
Title
Jia-Qi Liang; Qin Leng; Daianne F. Höfig; et al. Global and Planetary Change 2022. doi:10.1016/j.gloplacha.2022.103737
Other claims from this paper (16)
- Method / resource The modified cleared leaf method allowed accurate stomatal-size measurement.
- Observation Pore length was the most variable stomatal size parameter.
- Method / resource Whole-leaf stomatal density could be estimated accurately from a representative area in the middle of a leaf.
- Observation Within-leaf and between-leaf δ13C variation was only slightly above analytical error.
- Observation Early decay of Metasequoia leaf tissue produced an approximately 1‰ negative δ13C shift.
- Association The measured pore-length range had a major influence on model-estimated CO2.
- Association The measured whole-leaf stomatal-density range had a major influence on model-estimated CO2.
- Recommendation The authors recommend scaling pore length from guard cell length.
- Recommendation The authors suggest correcting carbon isotope fractionation when tissue decay and fossil preservation information is available.
- Method / resource Franks model CO2 estimates from 2004 modern leaves were 346 ppm versus a 378 ppm target.
- Method / resource Franks model CO2 estimates from 2020 modern leaves were 416 ppm versus a 414 ppm target.
- Method / resource Stomatal frequency methods underestimated modern CO2 at 285 ppm.
- Method / resource Stomatal frequency methods underestimated modern CO2 at 341 ppm.
- Method / resource Reconstructed CO2 for Metasequoia from the middle Miocene Clarkia deposit was 505 ppm, comparable with published results.
- Method / resource Reconstructed CO2 for Taxodium from the middle Miocene Clarkia deposit was 507 ppm, comparable with published results.
- Method / resource The improved leaf-clearing and stomatal-counting methods are expected to improve Franks model CO2 reconstruction performance.