Dehydrating apices after 0.4 M sucrose and 2 M glycerol reduced cryogenic procedure time compared with the conventional protocol by removing the 0.8 M sucrose pre-treatment.

Claim · model-written summary

Dehydrating apices after 0.4 M sucrose and 2 M glycerol reduced cryogenic procedure time compared with the conventional protocol by removing the 0.8 M sucrose pre-treatment.

Taxon

strawberryFragaria ×ananassat0

Treatment

a combination of 0.4 M sucrose and 2 M glycerole3

Outcome

time needed for the cryogenic proceduree4

Direction

reducede4

Qualifier

by eliminating the pre-treatment with 0.8 M sucrose for 19 he4

Comparator

conventional protocole3

Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

t0

“Apex cryopreservation of several strawberry genotypes”

e3

“Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol”

e4

“The latter method reduced considerably the time needed for the cryogenic procedure by eliminating the pre-treatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure”