Claim · model-written summary
The PEB adduct was photochemically inactive but fluorescent.
- Taxon
- oat
Avena sativat0 - Outcome
- photochemically inactivee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PEB adduct of ASPHYA-ST was photochemically inactive, it was intensely fluorescent with an excitation maximum at 576 nm and emission maxima at 586 nm.”
t0“Oat”
Title
Purification and Characterization of Recombinant Affinity Peptide‐Tagged Oat Phytochrome A
John T. Murphy; J. Clark Lagarias Photochemistry and Photobiology 1997. doi:10.1111/j.1751-1097.1997.tb01920.x
Other claims from this paper (9)
- Method / resource Full-length oat phytochrome A was purified to apparent homogeneity from yeast expression.
- Observation Tagged ASPHYA yielded a 124 kDa polypeptide.
- Method / resource ASPHYA-ST bound streptavidin agarose and eluted with 45% chromatographic efficiency.
- Observation P phi B and PCB adducts were photoactive.
- Observation The P phi B adduct had properties nearly indistinguishable from native photoreceptor.
- Observation The PCB adduct had blue-shifted absorption maxima.
- Observation PEB adduct excitation peaked at 576 nm.
- Observation PEB adduct emission peaked at 586 nm.
- Observation ASPHYA-ST and its three bilin adducts were homodimers.