Claim · model-written summary
The approach can be extended to other RNA viruses to identify homologous recombination hot spots.
- Taxon
- local lesion hoste0
- Outcome
- identification of homologous recombination hot spotse6
- Comparator
- other RNA virusese6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionValueQualifier
Verbatim
e0“Pairs of BMV RNA3 variants carrying marker mutations at different locations were coinoculated on a local lesion host, and the progeny RNA3 in a large number of lesions was analyzed”
e6“This approach can be easily extended to other RNA viruses for identification of homologous recombination hot spots”
Title
A. Bruyere; M. Wantroba; S. Flasinski; et al. Journal of Virology 2000. doi:10.1128/jvi.74.9.4214-4219.2000
Other claims from this paper (6)
- Observation The majority of doubly infected lesions accumulated RNA3 recombinants.
- Observation The distribution of recombinant types was relatively even, so both RNA3 counterparts could act as donor or acceptor.
- Observation Crossover frequency between closely marked RNA3 variants was similar to that between distantly marked variants.
- Observation The authors suggest an internal recombination hot spot in BMV RNA3.
- Observation Most crossovers were precise, while some recombinants had minor sequence modifications.
- Mechanism Discontinuous RNA replication, with the replicase switching templates, may generate RNA diversity.