Claim · model-written summary
The majority of doubly infected lesions accumulated RNA3 recombinants.
- Taxon
- local lesion hoste0
- Treatment
- coinoculatede0
- Interactor
- BMV
Brome mosaic viruse0pathogen - Outcome
- RNA3 recombinantse1
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pairs of BMV RNA3 variants carrying marker mutations at different locations were coinoculated on a local lesion host, and the progeny RNA3 in a large number of lesions was analyzed”
e1“The majority of doubly infected lesions accumulated the RNA3 recombinants”
Title
A. Bruyere; M. Wantroba; S. Flasinski; et al. Journal of Virology 2000. doi:10.1128/jvi.74.9.4214-4219.2000
Other claims from this paper (6)
- Observation The distribution of recombinant types was relatively even, so both RNA3 counterparts could act as donor or acceptor.
- Observation Crossover frequency between closely marked RNA3 variants was similar to that between distantly marked variants.
- Observation The authors suggest an internal recombination hot spot in BMV RNA3.
- Observation Most crossovers were precise, while some recombinants had minor sequence modifications.
- Mechanism Discontinuous RNA replication, with the replicase switching templates, may generate RNA diversity.
- Method / resource The approach can be extended to other RNA viruses to identify homologous recombination hot spots.