Claim · model-written summary
Most candidate proteins bound ZmWAKLY, with weak or absent interaction with ZmWAKLQ.
Zea maysNot reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most candidates, except for Zm00001d051405, bound to ZmWAKLY but showed weak or no interaction with ZmWAKLQ”
t0“maize”
Title
Tao Zhong; Chenyu Guo; Suining Deng; et al. Plant Biotechnology Journal 2025. doi:10.1111/pbi.70239
Other claims from this paper (19)
- Observation Fourteen proteins were identified as potential ZmWAKL interactors, including ZmHRL.
- Observation ZmHRL expression was induced by C. zeina infection.
- Observation ZmWAKLY interacted with ZmHRL in N. benthamiana.
- Observation ZmHRL bound the transmembrane and intracellular regions of ZmWAKLY.
- Observation ZmHRL was closest to AtHRL among maize HRL proteins, with 54.5% identity.
- Observation ZmHRL localized to the ER membrane in maize protoplasts.
- Observation ZmHRL overexpression lines showed increased transcript expression.
- Intervention effect ZmHRL overexpression enhanced GLS resistance with smaller lesions and lower severity than B73.
- Intervention effect ZmHRL overexpression reduced GLS scale in backcross populations relative to nontransgenic siblings.
- Intervention effect Zmhrl knockout mutants had significantly reduced GLS resistance relative to B73.
- Observation ZmHRL overexpression yielded 1086 upregulated genes.
- Observation ZmHRL overexpression yielded 1124 downregulated genes.
- Observation Oxidoreductase activity and calcium ion binding were enriched in GO analysis.
- Observation All nine oxidoreductase complex genes were downregulated.
- Observation Two oxidases and two peroxidases were upregulated.
- Observation Thirty-one calcium ion binding genes were upregulated, including twelve CPK genes.
- Observation Immune pathways were enriched in KEGG analysis.
- Intervention effect ZmHRL expression increased hydrogen peroxide accumulation in N. benthamiana.
- Observation ZmHRL overexpression raised flg22-triggered ROS while Zmhrl mutants reduced it relative to B73.