Claim · model-written summary
DAB staining of H2O2 was more intense in vascular bundle cells and in cortex cells.
- Taxon
- Mesembryanthemum crystallinum L.
Mesembryanthemum crystallinumt0 - Organ / stage
- vascular bundle cells and in cortex cellse0
- Outcome
- staining of H2O2e0
- Direction
- more intensee0
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mesembryanthemum crystallinum L.”
e0“3,3'-diaminobenzidine (DAB) staining of H2O2 was more intense in vascular bundle cells and in cortex cells”
Title
Marta Libik-Konieczny; Małgorzata Kozieradzka-Kiszkurno; Christine Desel; et al. PROTOPLASMA 2014. doi:10.1007/s00709-014-0692-2
Other claims from this paper (13)
- Mechanism Reactive oxygen species are involved in regulating rhizogenesis from hypocotyls of Mesembryanthemum crystallinum cultured on medium with NAA.
- Association Increased NADPH oxidase activity was correlated with increased hydrogen peroxide content and induction of mitotic activity in vascular cylinder cells of cultured hypocotyls.
- Intervention effect DPI, an NADPH oxidase inhibitor, inhibited H2O2 production in cultured Mesembryanthemum crystallinum hypocotyls.
- Intervention effect DPI, an NADPH oxidase inhibitor, blocked rhizogenesis in cultured Mesembryanthemum crystallinum hypocotyls.
- Observation Superoxide radical accumulation, shown by NBT precipitates, was localized within vascular cylinder cells in early rhizogenesis and at the tip of root primordia.
- Observation In newly formed roots H2O2 was localized in vascular tissue.
- Intervention effect Adding DPI to the medium decreased the intensity of NBT and DAB staining in cultured explants.
- Observation With DPI in the medium, O2 accumulation was limited to epidermis cells.
- Observation With DPI in the medium, H2O2 accumulated only in vascular tissue.
- Mechanism The authors suggest NADPH oxidase is responsible for regulating H2O2 level in vascular cylinder cells.
- Mechanism The authors suggest peroxidase participates in regulating H2O2 level in cortex cells.
- Mechanism The results indicate that O2 radical is engaged in rhizogenesis induction involving division of competent cells.
- Mechanism The results indicate that H2O2 is engaged in developmental processes mainly involving cell growth.