Claim · model-written summary
The protocol is stated to allow effective transformation and indirect regeneration of Brassica rapa.
- Taxon
- Brassica rapa
Brassica rapae6 - Outcome
- effective transformation and indirect regeneratione6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“This protocol allows effective transformation and indirect regeneration of Brassica rapa”
Title
Venkidasamy Baskar; Baniekal H. Gangadhar; Se Won Park; et al. 3 Biotech 2016. doi:10.1007/s13205-016-0402-1
Other claims from this paper (6)
- Intervention effect Pre-cultured hypocotyls from young seedlings showed higher shoot regeneration after exposure to Agrobacterium.
- Intervention effect A modest Agrobacterium concentration (0.8 OD) increased transformation efficiency in Chinese cabbage.
- Intervention effect A 3-day co-cultivation period increased transformation efficiency in Chinese cabbage.
- Intervention effect Plant growth hormones NAA and BAP were essential for callus and shoot formation in Chinese cabbage.
- Intervention effect Half-strength MS medium without root-inducing hormones was effective for root formation in Chinese cabbage.
- Method / resource The transformation protocol gave a transformation efficiency of 15% in Chinese cabbage.