Claim · model-written summary
The release of episomal replicons and the increase in GFP expression depended on the replication-associated protein Rep.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Gene
- replication-associated protein (Rep)e3
- Outcome
- increase in GFP expressione3
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Nicotiana benthamiana plants containing one copy of the cassette stably integrated into their genome were superinfected with TYLCSV, which mobilized and replicated the cassette as an episomal replicon.”
e3“The release of episomal trans replicons from the transgene and the increase in GFP expression were dependent on the cognate geminiviral replication-associated protein (Rep) and required interaction between Rep and the intergenic region of TYLCSV.”
Title
Gabriel Morilla; Araceli G. Castillo; Werner Preiss; et al. Journal of Virology 2006. doi:10.1128/jvi.80.7.3624-3633.2006
Other claims from this paper (6)
- Intervention effect Superinfection with TYLCSV increased green fluorescence in veins of leaves above the inoculation site, while GFP fluorescence was dimmed in intercostal areas.
- Mechanism Replication of TYLCSV required an interaction between Rep and the intergenic region of the virus.
- Method / resource The expression system is able to monitor the replication status of TYLCSV in plants, with GFP induced only in tissues where Rep is present.
- Intervention effect Inhibition of PCNA prevented GFP induction in veins.
- Intervention effect Inhibition of PCNA reduced viral DNA.
- Recommendation The authors propose that these plants could identify host factors required for geminivirus replication by virus-induced gene silencing.