Claim · model-written summary
The banana cDNA library had recombination rate of 100%.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Verbatim
e0“The average size of cDNA inserts ranged from 750 to 3 000 bp with a recombination rate of 100%.The rate of full-length gene was 68%.”
t0“Banana Roots”
Title
Xie JiangHui Zhongguo nongye Kexue 2011
Other claims from this paper (18)
- Method / resource Banana original cDNA library titer was 3.1×106 cfu·mL-1.
- Method / resource Banana amplified library titer was 2.8×108 cfu·mL-1.
- Method / resource The library yielded 421 unigenes, including 42% unknown genes, from 580 clones.
- Method / resource The banana cDNA library had size of cDNA inserts of 750 to 3 000 bp.
- Method / resource The banana cDNA library had rate of full-length gene of 68%.
- Observation The library provided protein genes associated with defense.
- Observation phenylalanine ammonialyase(PAL) expression was very low in non-inoculated banana.
- Observation catalase(CAT) expression was very low in non-inoculated banana.
- Observation β-1,3-glucanase(PR-2) expression was very low in non-inoculated banana.
- Observation pectin acetylesterase(PAE) expression was very low in non-inoculated banana.
- Intervention effect Seven defense genes increased after FOC race 4 inoculation.
- Observation peroxidase(POX) expression peaked on day six after FOC race 4 inoculation.
- Observation endochitinase(PR-3) expression peaked on day six after FOC race 4 inoculation.
- Observation PAE expression peaked on day six after FOC race 4 inoculation.
- Observation PAL expression peaked on day six after FOC race 4 inoculation.
- Observation Controls showed similar expression patterns but lower intensities for seven defense genes than inoculated plants.
- Method / resource The normalized full-length cDNA library was established for gene isolation.
- Mechanism Seven identified defense genes were interpreted to contribute to Fusarium wilt resistance.