Abstract
【Objective】The research purpose is to figure out the expression patterns of defense genes,screen candidate genes related to resistance to Fusarium wilt,and further clarify the resistance mechanism.【Method】A normalized cDNA enriched in full-length sequences was constructed from roots of banana cultivar Nongke No.1 inoculated with FOC race 4 using DSN(duplex-specific nuclease) normalization method combined with SMART(switching mechanism at 5′ end of the RNA transcript) technique.Furthermore,the expressions of defense genes were considered with multiplex semi-quantitative PCR.【Result】The liter of original cDNA library was 3.1×106 cfu·mL-1,and amplified library was 2.8×108 cfu·mL-1.Then,421 unigenes containing 42% unknown genes from the sequenced results of 580 clones selected randomly were obtained after the removal of low-quality sequences.The average size of cDNA inserts ranged from 750 to 3 000 bp with a recombination rate of 100%.The rate of full-length gene was 68%.Some protein genes associated with defense were obtained on the basis of cDNA library.By RT-PCR analysis,the expression levels of phenylalanine ammonialyase(PAL),catalase(CAT),β-1,3-glucanase(PR-2) and pectin acetylesterase(PAE) in non-inoculated condition were very low.However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.Though the expression patterns of seven defense genes were similar with the controls,the intensities were much less than the inoculations.【Conclusion】 These results indicated that the normalized full-length cDNA library was established successfully,which can be used for isolating required gene in the light of the rate of recombinant and integrity.Meanwhile,seven defense genes were identified,which played a key role in resistance to Fusarium wilt in some extent.
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Claims 19
Claim · model-written summary
Banana original cDNA library titer was 3.1×106 cfu·mL-1.
Verbatim
e0“The liter of original cDNA library was 3.1×106 cfu·mL-1,and amplified library was 2.8×108 cfu·mL-1.”
t0“Banana Roots”
Claim · model-written summary
Banana amplified library titer was 2.8×108 cfu·mL-1.
Verbatim
e0“The liter of original cDNA library was 3.1×106 cfu·mL-1,and amplified library was 2.8×108 cfu·mL-1.”
t0“Banana Roots”
Claim · model-written summary
The library yielded 421 unigenes, including 42% unknown genes, from 580 clones.
Verbatim
e0“Then,421 unigenes containing 42% unknown genes from the sequenced results of 580 clones selected randomly were obtained after the removal of low-quality sequences.”
t0“Banana Roots”
Claim · model-written summary
The banana cDNA library had size of cDNA inserts of 750 to 3 000 bp.
Verbatim
e0“The average size of cDNA inserts ranged from 750 to 3 000 bp with a recombination rate of 100%.The rate of full-length gene was 68%.”
t0“Banana Roots”
Claim · model-written summary
The banana cDNA library had recombination rate of 100%.
Verbatim
e0“The average size of cDNA inserts ranged from 750 to 3 000 bp with a recombination rate of 100%.The rate of full-length gene was 68%.”
t0“Banana Roots”
Claim · model-written summary
The banana cDNA library had rate of full-length gene of 68%.
Verbatim
e0“The average size of cDNA inserts ranged from 750 to 3 000 bp with a recombination rate of 100%.The rate of full-length gene was 68%.”
t0“Banana Roots”
Claim · model-written summary
The library provided protein genes associated with defense.
Verbatim
e0“Some protein genes associated with defense were obtained on the basis of cDNA library.”
t0“Banana Roots”
Claim · model-written summary
phenylalanine ammonialyase(PAL) expression was very low in non-inoculated banana.
Verbatim
e0“By RT-PCR analysis,the expression levels of phenylalanine ammonialyase(PAL),catalase(CAT),β-1,3-glucanase(PR-2) and pectin acetylesterase(PAE) in non-inoculated condition were very low.”
t0“Banana Roots”
Claim · model-written summary
catalase(CAT) expression was very low in non-inoculated banana.
Verbatim
e0“By RT-PCR analysis,the expression levels of phenylalanine ammonialyase(PAL),catalase(CAT),β-1,3-glucanase(PR-2) and pectin acetylesterase(PAE) in non-inoculated condition were very low.”
t0“Banana Roots”
Claim · model-written summary
β-1,3-glucanase(PR-2) expression was very low in non-inoculated banana.
Verbatim
e0“By RT-PCR analysis,the expression levels of phenylalanine ammonialyase(PAL),catalase(CAT),β-1,3-glucanase(PR-2) and pectin acetylesterase(PAE) in non-inoculated condition were very low.”
t0“Banana Roots”
Claim · model-written summary
pectin acetylesterase(PAE) expression was very low in non-inoculated banana.
Verbatim
e0“By RT-PCR analysis,the expression levels of phenylalanine ammonialyase(PAL),catalase(CAT),β-1,3-glucanase(PR-2) and pectin acetylesterase(PAE) in non-inoculated condition were very low.”
t0“Banana Roots”
Claim · model-written summary
Seven defense genes increased after FOC race 4 inoculation.
- Taxon
- Bananat0
- Treatment
- inoculated by FOC race 4e0
- Interactor
- FOC race 4e0pathogen
- Gene
- seven defense genese0
- Outcome
- seven defense genese0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.”
t0“Banana Roots”
Claim · model-written summary
peroxidase(POX) expression peaked on day six after FOC race 4 inoculation.
Verbatim
e0“However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.”
t0“Banana Roots”
Claim · model-written summary
endochitinase(PR-3) expression peaked on day six after FOC race 4 inoculation.
Verbatim
e0“However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.”
t0“Banana Roots”
Claim · model-written summary
PAE expression peaked on day six after FOC race 4 inoculation.
Verbatim
e0“However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.”
t0“Banana Roots”
Claim · model-written summary
PAL expression peaked on day six after FOC race 4 inoculation.
Verbatim
e0“However,seven defense genes were obviously increased after inoculated by FOC race 4,and peroxidase(POX),endochitinase(PR-3),PAE and PAL reached the peak in the sixth day.”
t0“Banana Roots”
Claim · model-written summary
Controls showed similar expression patterns but lower intensities for seven defense genes than inoculated plants.
Verbatim
e0“Though the expression patterns of seven defense genes were similar with the controls,the intensities were much less than the inoculations.”
t0“Banana Roots”
Claim · model-written summary
The normalized full-length cDNA library was established for gene isolation.
Verbatim
e0“These results indicated that the normalized full-length cDNA library was established successfully,which can be used for isolating required gene in the light of the rate of recombinant and integrity.Meanwhile,seven defense genes were identified,which played a key role in resistance to Fusarium wilt in some extent.”
t0“Banana Roots”
Claim · model-written summary
Seven identified defense genes were interpreted to contribute to Fusarium wilt resistance.
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicated that the normalized full-length cDNA library was established successfully,which can be used for isolating required gene in the light of the rate of recombinant and integrity.Meanwhile,seven defense genes were identified,which played a key role in resistance to Fusarium wilt in some extent.”
t0“Banana Roots”
The same questions in other papers
inoculation · molecular: expression · MusaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.