Claim · model-written summary
Unique substitutions (singletons) remained constant in frequency during passage regardless of host species.
- Taxon
- wheat, corn, and barley
Hordeum vulgare
Triticum aestivumZea mays subsp. mayse3
- Outcome
- unique substitutions (singletons)e4
- Direction
- remained constante4
- Qualifier
- regardless of the host species examinede4
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e3“to establish three lineages each in wheat, corn, and barley”
e4“a pool of unique substitutions (singletons) that remained constant in frequency during passage, regardless of the host species examined”
Title
Structure and Temporal Dynamics of Populations within Wheat Streak Mosaic Virus Isolates
Jeffrey S. Hall; Roy French; T. Jack Morris; et al. Journal of Virology 2001. doi:10.1128/jvi.75.21.10231-10243.2001
Other claims from this paper (9)
- Observation SSCP patterns among limiting-dilution subisolates were associated with single-nucleotide substitutions.
- Observation By passage 9, each WSMV lineage differed in consensus sequence from the founding isolate LDSI-S10.
- Observation Most substitutions occurred within NIa and CP.
- Observation Most consensus sequence changes among lineages were independent, with substitutions accumulating over time.
- Observation The variant nucleotide G(6016) in NIa was restored to A(6016) in eight of nine lineages by passage 6.
- Mechanism The near-global reversion of the NIa nucleotide is most easily explained by selection.
- Observation Nonsingleton variation increased upon passage.
- Mechanism Mutations from viral polymerase error arise at a constant rate, but most new mutants are sequestered in virions and are not replication templates.
- Observation A substantial fraction of the variation generated is static and untested for relative fitness.