Claim · model-written summary
Both barley amylase isoform groups required one calcium atom per enzyme molecule.
- Taxon
- barley
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Hordeum vulgaret0 - Gene
- α-amylaset0
- Outcome
- required one atom of Ca2+/molecule of enzymee0
- Value
- one atom of Ca2+/molecule of enzymee0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Both groups of isoforms required one atom of Ca2+/molecule of enzyme”
t0“barley aleurone α-amylase”
Title
D S Bush; L Sticher; R van Huystee; et al. Journal of Biological Chemistry 1989. doi:10.1016/s0021-9258(19)47314-6
Other claims from this paper (5)
- Observation The two barley amylase groups differed in calcium affinity by more than tenfold.
- Intervention effect Low calcium irreversibly inactivated both barley amylase groups.
- Mechanism Inactivation appeared linked to changes in secondary and tertiary structure, not primary structure.
- Observation The predicted calcium binding region of barley amylase resembles that of mammalian amylase.
- Mechanism Micromolar calcium is required to stabilize barley amylases in the aleurone endoplasmic reticulum.