Claim · model-written summary
After 2-mercaptoethanol treatment only 2 bands were found, at Mr = 29,000 and 34,000.
- Taxon
- Viscum album
Viscum albumt0 - Treatment
- 2-mercaptoethanole4
- Outcome
- only 2 bandse4
- Value
- Mr = 29,000 and 34,000e4
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Viscum album L. (mistletoe)”
e4“After treatment with 2-mercaptoethanol, only 2 bands (Mr = 29,000 and 34,000) were found”
Title
Isolation and characterization of viscumin, a toxic lectin from Viscum album L. (mistletoe).
S Olsnes; F Stirpe; K Sandvig; et al. Journal of Biological Chemistry 1982. doi:10.1016/s0021-9258(18)33440-9
Other claims from this paper (8)
- Observation Viscumin migrated at Mr = 60,000 in SDS polyacrylamide gel electrophoresis.
- Observation Viscumin was selectively bound to the affinity column and eluted with lactose.
- Observation Viscumin consists of two chains, which are in some molecules disulfide-linked.
- Observation The major part of the cytotoxic activity in mistletoe extracts is due to viscumin.
- Observation Viscumin occurs as a monomer at low concentrations and as a dimer at higher concentrations.
- Observation Viscumin inhibits protein synthesis in cell-free systems.
- Mechanism The A chain inhibits protein synthesis by inactivating ribosomes catalytically.
- Intervention effect The A chain-treated ribosomes showed selective inactivation of the 60 S ribosomal subunit relative to untreated ribosomes.