Claim · model-written summary
A low concentration of ZT was advantageous to induction of cotton embryogenic callus.
- Taxon
- Gossypium hirsutum L.
Gossypium hirsutume0 - Treatment
- low concentration of ZTe4
- Outcome
- induction of embryogenic calluse4
- Direction
- advantageouse4
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An elite Chinese cotton (Gossypium hirsutum L.) cultivar Simian-3 was chosen for tissue culture”
e4“a low concentration of ZT was advantageous to the induction of embryogenic callus”
Title
High Frequency Somatic Embryogenesis and Plant Regeneration of an Elite Chinese Cotton Variety
Baohong Zhang; Rong Feng; Fang Liu; et al. Zhōngyāng yánjiūyuàn zhíwùxué huikān/Zhōngyāng yánjiūyuàn zhíwùxué huikān 2001
Other claims from this paper (12)
- Association Cotton hypocotyl explants gave much easier callus induction than cotyledon or root explants.
- Association The optimum ZT concentration for cotton callus induction was 3.0~5.0 mg/L.
- Association Root explants were the most responsive for cotton somatic embryo production, then hypocotyl, then cotyledon.
- Intervention effect 2,4-D promoted proliferation of embryogenic callus in cotton.
- Intervention effect 2,4-D had a negative effect on differentiation and germination of cotton somatic embryos.
- Intervention effect Activated charcoal could promote production, maturation and germination of cotton somatic embryos.
- Intervention effect A combination of ZT and IAA could promote production, maturation and germination of cotton somatic embryos.
- Method / resource ZH medium with 2,4-D, kinetin and ZT was the best medium for proliferation of cotton embryogenic callus.
- Method / resource MSB with ZT and activated charcoal was the best medium for differentiation and germination of cotton somatic embryos.
- Observation Two kinds of callus, embryogenic and nonembryogenic, were identified after 70 days of cotton culture.
- Observation Embryogenic cotton callus developed into somatic embryos after 20 days of subculture.
- Observation Complete cotton plants could be regenerated via somatic embryogenesis from hypocotyl, cotyledon and root explants in 3-4 months.