Claim · model-written summary
dsRNA gels showed viral genome and subgenomic RNA bands.
- Outcome
- strong visible bands corresponding to high-molecular-weight virus genome and to subgenomic RNAe0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“DsRNA extractions separated on agarose gels showed strong visible bands corresponding to high-molecular-weight virus genome and to subgenomic RNA”
Title
Cord Mikona; Wilhelm Jelkmann Plant Disease 2010. doi:10.1094/pdis-94-4-0471
Other claims from this paper (12)
- Observation Cuscuta reflexa transmitted GLRaV-7 from grapevine to Tetragonia expansa.
- Observation Cuscuta europea transmitted GLRaV-7 to Nicotiana occidentalis.
- Observation Cuscuta campestris became infected with GLRaV-7.
- Observation Cuscuta campestris could not transfer the virus to an experimental host.
- Method / resource RT-PCR verified viral transmission from all five plant species.
- Observation GLRaV-7 persisted in four experimental plant species for more than four years.
- Observation Infected T. expansa and Cuscuta species remained symptomless.
- Observation Infected N. occidentalis developed severe symptoms, stunting and decline.
- Observation GLRaV-7 titers differed greatly between tissues of natural and experimental hosts.
- Observation Virus replication was demonstrated in Cuscuta.
- Method / resource Experimental hosts and Cuscuta allowed dsRNA extraction for viral genome characterization.
- Recommendation The alternative host plants facilitate nucleic acid extraction and could serve as etiological models.