Claim · model-written summary
No cross-reactivity occurred with antifibronectin receptor antiserum.
- Taxon
- Soybean
Glycine maxe2 - Gene
- antifibronectin receptore0
- Outcome
- no cross-reactivitye0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no cross-reactivity was observed with antifibronectin receptor antiserum.”
e1“no cross-reactivity was observed with antifibronectin receptor antiserum.”
t0“plant cell wall and plasma membrane”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)”
Title
RGD-dependent linkage between plant cell wall and plasma membrane: consequences for growth.
M Schindler; S Meiners; D A Cheresh The Journal of Cell Biology 1989. doi:10.1083/jcb.108.5.1955
Other claims from this paper (7)
- Intervention effect GRGDSP significantly enhanced soybean cell growth rate.
- Intervention effect GRGDSP caused aberrant soybean cell wall and plasma membrane organization.
- Intervention effect Asp-to-Glu substitution or RGD-to-GDR inversion abolished the peptide morphological and growth effects.
- Observation Anti-hVNR immunoblots detected one 70-72 kD band in soybean cells and protoplasts.
- Observation Affinity chromatography retained one 70-72 kD polypeptide reactive with anti-hVNR antiserum.
- Observation Fluorescent patches occurred at the soybean cell membrane-wall boundary.
- Mechanism The authors propose an RGD-dependent recognition system mediates cell division and wall biosynthesis.