Abstract
Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate, and (b) aberrant cell wall/plasma membrane interactions and organization. Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells. Immunoblots, which were prepared from beta-octylglucoside extracts of whole soybean cells and protoplasts, probed with polyclonal antibodies raised against human vitronectin receptor (hVNR) complex, demonstrated a single band with an apparent molecular mass of 70-72 kD. Chromatography of beta-octylglucoside extracts of SB-1 cells on a Gly-Arg-Gly-Asp-Ser-Pro-Lys-Sepharose affinity column demonstrated the retention of a single 70-72 kD polypeptide that reacted specifically with anti-hVNR antiserum. In contradistinction, no cross-reactivity was observed with antifibronectin receptor antiserum. Epifluorescence microscopy of whole soybean cells, after moderate treatment with pectinase, demonstrated punctate fluorescent patches at the cell membrane/wall boundary when probed with anti-hVNR and rhodamine-derivatized secondary antibodies. We propose that coordination and control of plant cell division and proper cell wall biosynthesis may be mediated by an RGD-dependent recognition system in which RGD binding protein(s) promote cell membrane-cell wall attachment.
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Claims 8
Claim · model-written summary
GRGDSP significantly enhanced soybean cell growth rate.
- Taxon
- Soybean
Glycine maxe0 - Material
- SB-1 cell linee0
- Treatment
- Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)e0
- Outcome
- growth ratee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate”
e1“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate”
Claim · model-written summary
GRGDSP caused aberrant soybean cell wall and plasma membrane organization.
- Taxon
- SoybeanGlycine maxe2
- Treatment
- GRGDSPe2
- Outcome
- aberrant cell wall/plasma membrane interactions and organizatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“(b) aberrant cell wall/plasma membrane interactions and organization.”
e1“(b) aberrant cell wall/plasma membrane interactions and organization.”
t0“plant cell wall and plasma membrane”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)”
Claim · model-written summary
Asp-to-Glu substitution or RGD-to-GDR inversion abolished the peptide morphological and growth effects.
- Taxon
- SoybeanGlycine maxe2
- Treatment
- Substitution of the Asp (D) by a Glu (E) amino acide0
- Outcome
- morphological and growth effectse0
- Direction
- abolishede0
- Comparator
- GRGDSPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells.”
e1“Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells.”
t0“plant cell wall and plasma membrane”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)”
Claim · model-written summary
Anti-hVNR immunoblots detected one 70-72 kD band in soybean cells and protoplasts.
- Taxon
- soybeanGlycine maxe0
- Outcome
- a single bande0
- Value
- 70-72 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoblots, which were prepared from beta-octylglucoside extracts of whole soybean cells and protoplasts, probed with polyclonal antibodies raised against human vitronectin receptor (hVNR) complex, demonstrated a single band with an apparent molecular mass of 70-72 kD.”
e1“Immunoblots, which were prepared from beta-octylglucoside extracts of whole soybean cells and protoplasts, probed with polyclonal antibodies raised against human vitronectin receptor (hVNR) complex, demonstrated a single band with an apparent molecular mass of 70-72 kD.”
Claim · model-written summary
Affinity chromatography retained one 70-72 kD polypeptide reactive with anti-hVNR antiserum.
- Taxon
- SoybeanGlycine maxe2
- Outcome
- retention of a single 70-72 kD polypeptidee0
- Value
- 70-72 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“demonstrated the retention of a single 70-72 kD polypeptide that reacted specifically with anti-hVNR antiserum.”
e1“demonstrated the retention of a single 70-72 kD polypeptide that reacted specifically with anti-hVNR antiserum.”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line)”
Claim · model-written summary
No cross-reactivity occurred with antifibronectin receptor antiserum.
- Taxon
- SoybeanGlycine maxe2
- Gene
- antifibronectin receptore0
- Outcome
- no cross-reactivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no cross-reactivity was observed with antifibronectin receptor antiserum.”
e1“no cross-reactivity was observed with antifibronectin receptor antiserum.”
t0“plant cell wall and plasma membrane”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)”
Claim · model-written summary
Fluorescent patches occurred at the soybean cell membrane-wall boundary.
- Taxon
- soybeanGlycine maxe0
- Outcome
- punctate fluorescent patches at the cell membrane/wall boundarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Epifluorescence microscopy of whole soybean cells, after moderate treatment with pectinase, demonstrated punctate fluorescent patches at the cell membrane/wall boundary when probed with anti-hVNR and rhodamine-derivatized secondary antibodies.”
e1“Epifluorescence microscopy of whole soybean cells, after moderate treatment with pectinase, demonstrated punctate fluorescent patches at the cell membrane/wall boundary when probed with anti-hVNR and rhodamine-derivatized secondary antibodies.”
Claim · model-written summary
The authors propose an RGD-dependent recognition system mediates cell division and wall biosynthesis.
- Taxon
- SoybeanGlycine maxe2
- Outcome
- coordination and control of plant cell division and proper cell wall biosynthesise0
- Qualifier
- RGD-dependent recognition systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that coordination and control of plant cell division and proper cell wall biosynthesis may be mediated by an RGD-dependent recognition system in which RGD binding protein(s) promote cell membrane-cell wall attachment.”
e1“We propose that coordination and control of plant cell division and proper cell wall biosynthesis may be mediated by an RGD-dependent recognition system in which RGD binding protein(s) promote cell membrane-cell wall attachment.”
e2“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line)”
The same questions in other papers
amino acid · growth · Glycine max (soybean)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.