Paper · 1989 · ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/87/0d/J_Cell_Biol_1989_May_1_108(5)_1955-1965.tar.gz

RGD-dependent Linkage between Plant Cell Wall and Plasma Membrane: Consequences for Growth

Melvin Schindler; Sally Meiners; David A. Cheresh

4
claims
6
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Abstract. Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate, and (b) aberrant cell wall/plasma membrane interactions and organization. Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells. Immunoblots, which were prepared from ~-octylglucoside extracts of whole soybean cells and protoplasts, probed with polyclonal antibodies raised

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Claims 4

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

GRGDSP significantly enhanced soybean root cell growth rate.

Taxon
SoybeanGlycine maxe0
Material
SB-1 cell linee0
Organ / stage
root cellse0
Treatment
Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)e0
Outcome
growth ratee0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate”

✓ located verbatim in the source · abstract, characters 10–344 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

GRGDSP caused aberrant cell wall and plasma membrane interactions and organization.

Taxon
SoybeanGlycine maxe0
Treatment
Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml)e0
Outcome
aberrant cell wall/plasma membrane interactions and organizatione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells (SB-1 cell line) grown in suspension culture containing Glycyl-Arginyl-Glycyl-Aspartyl-Seryl-Proline (GRGDSP) (0.25 mg/ml), a synthetic peptide containing the RGD sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate, and (b) aberrant cell wall/plasma membrane interactions and organization”

✓ located verbatim in the source · abstract, characters 10–418 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Asp to Glu substitution abolished the morphological and growth effects of GRGDSP.

Taxon
SoybeanGlycine maxe1
Treatment
Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptidee0
Outcome
morphological and growth effectse0
Direction
abolishede0
Comparator
GRGDSPe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells”

✓ located verbatim in the source · abstract, characters 420–614 · show in text ↑
e1

“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells”

✓ located verbatim in the source · abstract, characters 10–66 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

RGD inversion to GDR abolished the morphological and growth effects of GRGDSP.

Taxon
SoybeanGlycine maxe1
Treatment
inversion of the RGD sequence to GDRe0
Outcome
morphological and growth effectse0
Direction
abolishede0
Comparator
GRGDSPe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Substitution of the Asp (D) by a Glu (E) amino acid in the hexapeptide, or inversion of the RGD sequence to GDR, abolished the morphological and growth effects observed for GRGDSP in plant cells”

✓ located verbatim in the source · abstract, characters 420–614 · show in text ↑
e1

“Soybean (Glycine max [L.] Merr. cv. Mandarin) root cells”

✓ located verbatim in the source · abstract, characters 10–66 · show in text ↑

amino acid · growth · Glycine max (soybean)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. no effect
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.