Claim · model-written summary
Extra lipo-chitooligosaccharides suppressed cell death induced by growth-regulator withdrawal.
- Treatment
- extra supply of lipo-chitooligosaccharides (LCOs)e0
- Outcome
- Cell deathe0
- Direction
- suppressede0
- Qualifier
- withdrawal of PGRse0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValue
Verbatim
e0“Cell death, induced by withdrawal of PGRs, can be suppressed by extra supply of lipo-chitooligosaccharides (LCOs).”
Title
Propagation of Norway spruce via somatic embryogenesis
Sara von Arnold; Peter V. Bozhkov; David E. Clapham; et al. Springer Netherlands 2005. doi:10.1007/1-4020-3200-5-20
Other claims from this paper (7)
- Observation Norway spruce embryogenic cell lines proliferated as proembryogenic masses, from which somatic embryos developed.
- Intervention effect Withdrawal of plant growth regulators stimulated the transition from proembryogenic masses to somatic embryos and accompanied programmed cell death.
- Mechanism A marked decrease in extracellular pH mediated programmed cell death in the proembryogenic masses.
- Intervention effect Buffering the culture medium inhibited the transition to somatic embryos and programmed cell death.
- Association Formation of the embryo surface layer accompanied a shift of Pa18 and PaHB1 expression from ubiquitous in proembryogenic masses to the surface layer in somatic embryos.
- Intervention effect Ectopic expression of Pa18 and PaHB1 caused an early developmental block.
- Method / resource The biolistic approach routinely produced transgenic Norway spruce embryos and plants.