Claim · model-written summary
ER-associated enzyme stayed membrane-integrated after detergent or Mg++ depletion.
- Taxon
- tobacco plants
Nicotiana tabacumt0 - Treatment
- detergent or Mg++-depletione0
- Outcome
- firmly integrated into membrane structuree0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was firmly integrated into membrane structure, even after treated with detergent or Mg++-depletion”
t0“tobacco plants”
Title
Yoichi TAKANAMI; Naoto NITTA; Susumu KUBO Japanese Journal of Phytopathology 1989. doi:10.3186/jjphytopath.55.635
Other claims from this paper (9)
- Observation Membrane-bound polymerase activity increased before virus multiplication.
- Observation Soluble polymerase activity rose rapidly after CMV concentration peaked.
- Observation In vitro M-RDRP products were mainly double-stranded CMV RNAs.
- Observation M-RDRP was inferred to associate with endoplasmic reticulum.
- Observation ER-associated enzyme had viral RNAs as endogenous templates.
- Method / resource Purification effectively removed soluble polymerase.
- Observation S-RDRP synthesized small heterogeneous RNA in vitro.
- Mechanism S-RDRP was suggested to be a host enzyme induced in infected leaf tissue but not protoplasts.
- Mechanism Authors speculated that CMV RNA replication complex includes viral translation products, ER membranes and viral template RNAs.