Abstract
Activity of membrane-bound RNA-dependent RNA polymerase (M-RDRP) from cucumber mosaic virus (CMV)-infected tobacco plants increased prior to virus multiplication and that of soluble enzyme (S-RDRP) increased rapidly after concentration of CMV has reached its maximum. In vitro products of M-RDRP was mainly doublestranded CMV RNAs as replicative forms. M-RDRP was suggested to be associated with endoplasmic reticulum (ER) by subcellular fractionation of the membrane-fraction using Percoll discontinuous gradient-centrifugation and electron microscopic observation. The ER-associated enzyme had viral RNAs as endogenous templates and was firmly integrated into membrane structure, even after treated with detergent or Mg++-depletion or partially purified by Sepharose 4B chromatography. The purification procedures could effectively remove S-RDRP which synthesized small heterogeneous RNA in vitro. Joined with our previous study using CMV-infected tobacco protoplasts, it was suggested that S-RDRP is a host-encoded enzyme induced in leaf tissues but not in protoplasts as a consequence of virus infection process like “Pathogenesis-related proteins.” We speculate that the CMV RNA replication complex is composed at least of translation products of viral RNAs, ER membranes and viral template RNAs.
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Claims 10
Claim · model-written summary
Membrane-bound polymerase activity increased before virus multiplication.
- Taxon
- tobacco plants
Nicotiana tabacume0 - Interactor
- cucumber mosaic virus (CMV)
cucumber mosaic cucumoviruse0pathogen - Gene
- membrane-bound RNA-dependent RNA polymerase (M-RDRP)e0
- Outcome
- Activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity of membrane-bound RNA-dependent RNA polymerase (M-RDRP) from cucumber mosaic virus (CMV)-infected tobacco plants increased prior to virus multiplication”
Claim · model-written summary
Soluble polymerase activity rose rapidly after CMV concentration peaked.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- soluble enzyme (S-RDRP)e0
- Outcome
- that of soluble enzyme (S-RDRP)e0
- Direction
- increased rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“that of soluble enzyme (S-RDRP) increased rapidly after concentration of CMV has reached its maximum.”
t0“tobacco plants”
Claim · model-written summary
In vitro M-RDRP products were mainly double-stranded CMV RNAs.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- M-RDRPe0
- Outcome
- doublestranded CMV RNAs as replicative formse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro products of M-RDRP was mainly doublestranded CMV RNAs as replicative forms.”
t0“tobacco plants”
Claim · model-written summary
M-RDRP was inferred to associate with endoplasmic reticulum.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Gene
- M-RDRPe0
- Outcome
- associated with endoplasmic reticulum (ER)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“M-RDRP was suggested to be associated with endoplasmic reticulum (ER)”
t0“tobacco plants”
Claim · model-written summary
ER-associated enzyme had viral RNAs as endogenous templates.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- ERe0
- Outcome
- viral RNAs as endogenous templatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ER-associated enzyme had viral RNAs as endogenous templates”
t0“tobacco plants”
Claim · model-written summary
ER-associated enzyme stayed membrane-integrated after detergent or Mg++ depletion.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Treatment
- detergent or Mg++-depletione0
- Outcome
- firmly integrated into membrane structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was firmly integrated into membrane structure, even after treated with detergent or Mg++-depletion”
t0“tobacco plants”
Claim · model-written summary
Purification effectively removed soluble polymerase.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Gene
- S-RDRPe0
- Outcome
- effectively remove S-RDRPe0
- Comparator
- purification procedurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purification procedures could effectively remove S-RDRP which synthesized small heterogeneous RNA in vitro.”
t0“tobacco plants”
Claim · model-written summary
S-RDRP synthesized small heterogeneous RNA in vitro.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Gene
- S-RDRPe0
- Outcome
- small heterogeneous RNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-RDRP which synthesized small heterogeneous RNA in vitro.”
t0“tobacco plants”
Claim · model-written summary
S-RDRP was suggested to be a host enzyme induced in infected leaf tissue but not protoplasts.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Organ / stage
- leaf tissuese0
- Interactor
- viruse0pathogen
- Gene
- S-RDRPe0
- Outcome
- host-encoded enzyme induced in leaf tissues but not in protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-RDRP is a host-encoded enzyme induced in leaf tissues but not in protoplasts as a consequence of virus infection process”
t0“tobacco plants”
Claim · model-written summary
Authors speculated that CMV RNA replication complex includes viral translation products, ER membranes and viral template RNAs.
- Taxon
- tobacco plantsNicotiana tabacumt0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- CMV RNA replication complexe0
- Qualifier
- translation products of viral RNAs, ER membranes and viral template RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We speculate that the CMV RNA replication complex is composed at least of translation products of viral RNAs, ER membranes and viral template RNAs.”
t0“tobacco plants”