Claim · model-written summary
The rpiA gene has a key role in suppression of damping-off and in colonization of subterranean plant portions by E. cloacae, possibly through the pentose phosphate pathway.
- Taxon
- cucumber
Cucumis sativuse0 - Gene
- rpiAe7
- Outcome
- suppression of damping-offe7
- Qualifier
- possiblye7
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strains of Enterobacter cloacae show promise as biocontrol agents for Pythium ultimum-induced damping-off on cucumber and other crops.”
e7“Experiments reported here indicate a key role for rpiA and possibly the pentose phosphate pathway in suppression of damping-off and colonization of subterranean portions of plants by E. cloacae”
Title
Scott M. Lohrke; Pierre D. Dery; Wei Li; et al. Molecular Plant-Microbe Interactions 2002. doi:10.1094/mpmi.2002.15.8.817
Other claims from this paper (10)
- Intervention effect The mini-Tn5 Km mutant A145 of E. cloacae was significantly reduced in suppressing cucumber damping-off caused by P. ultimum relative to parent strain 501R3.
- Intervention effect Strain A145 was deficient in seed colonization of cucumber, sunflower and wheat relative to strain 501R3.
- Intervention effect Strain A145 was significantly reduced in seed colonization of corn and cowpea relative to strain 501R3.
- Intervention effect Populations of strain A145 were significantly lower than those of strain 501R3 at all sampling times in cucumber, wheat and sunflower rhizosphere.
- Observation Populations of strain A145 were not detectable in any rhizosphere after 42 days.
- Observation Mini-Tn5 Km inserted into a region of the E. cloacae genome with high DNA and amino acid similarity to rpiA.
- Intervention effect Ribose-5-phosphate isomerase activity in cell lysates of strain A145 was about 3.5% of that of strain 501R3.
- Observation Strain A145 was a ribose auxotroph, as expected for an rpiA mutant.
- Intervention effect Introducing the rpiA homologue fragment into strain A145 restored its ribose phosphate isomerase activity, prototrophy and seedling colonization to levels similar to strain 501R3.
- Intervention effect Introducing the rpiA homologue fragment into strain A145 restored disease suppression to levels similar to strain 501R3.