Claim · model-written summary
Gly-Gly uptake was sensitive to thiol reagents and metabolic inhibitors.
- Taxon
- broad bean
Vicia fabat0 - Treatment
- thiol reagents and metabolic inhibitorse0
- Outcome
- sensitivee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sensitive to thiol reagents and metabolic inhibitors”
t0“broad bean”
Title
Proton-Peptide Co-Transport in Broad Bean Leaf Tissues
A. Jamai; J. F. Chollet; S. Delrot PLANT PHYSIOLOGY 1994. doi:10.1104/pp.106.3.1023
Other claims from this paper (15)
- Observation Gly-Gly transport rate was similar to Gly uptake in glycine equivalents.
- Observation Gly-Gly uptake occurred mainly in mesophyll cells, with little vein accumulation.
- Observation Gly-Gly uptake was optimal at pH 6.0.
- Observation Gly-Gly uptake had one saturable phase with an apparent Michaelis constant of 16 mM.
- Intervention effect Gly-Gly did not inhibit labeled Gly uptake.
- Intervention effect Gly-Gly caused a concentration-dependent rise in medium pH.
- Intervention effect Gly-Gly induced concentration-dependent mesophyll depolarization with an apparent Michaelis constant of 15 mM.
- Observation Depolarization was followed by transient hyperpolarization.
- Intervention effect Several peptides and tripeptides inhibited Gly-Gly uptake at tenfold excess.
- Intervention effect Gly only slightly inhibited Gly-Gly uptake.
- Intervention effect TetraGly did not inhibit Gly-Gly uptake.
- Intervention effect Glutathione did not inhibit Gly-Gly uptake.
- Intervention effect Dipeptides that inhibited Gly-Gly uptake changed mesophyll membrane potential.
- Intervention effect Dipeptides changed the membrane response normally induced by Gly-Gly.
- Mechanism The data indicate a low-affinity, broad-specificity H+/peptide cotransporter in mesophyll plasma membrane.