Claim · model-written summary
Deleting residues 240 to 242 dramatically impaired cell-to-cell movement of Bamboo mosaic virus.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- Deletion of residues 240 to 242e2
- Interactor
- Bamboo mosaic virus
.jpg?width=60)
Bamboo mosaic viruse0pathogen - Outcome
- cell-to-cell movemente3
- Direction
- impairede3
- Qualifier
- dramaticallye3
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e2“Deletion of residues 240 to 242 increased the affinity of CP to viral RNA”
e3“dramatically impaired cell-to-cell movement”
e1“Nicotiana benthamiana”
e0“Bamboo mosaic virus”
Title
Chien-Jen Hung; Ying-Wen Huang; Ming-Ru Liou; et al. Molecular Plant-Microbe Interactions 2014. doi:10.1094/mpmi-04-14-0112-r
Other claims from this paper (10)
- Observation The coat protein of Bamboo mosaic virus is phosphorylated in Nicotiana benthamiana at serine 241 by NbCK2α.
- Association The coat protein of Bamboo mosaic virus and NbCK2α colocalize at the plasmodesmata, suggesting a possible role of phosphorylation in virus movement.
- Intervention effect Replacing S241 with alanine did not affect RNA binding affinity of Bamboo mosaic virus coat protein in Nicotiana benthamiana.
- Intervention effect Replacing S241 with alanine moderately impaired cell-to-cell movement of Bamboo mosaic virus in Nicotiana benthamiana.
- Intervention effect A negative charge at position 241 reduced the ability of the coat protein to bind RNA.
- Intervention effect A negative charge at position 241 severely interfered with cell-to-cell movement of Bamboo mosaic virus.
- Intervention effect Deleting residues 240 to 242 increased the affinity of the coat protein to viral RNA.
- Intervention effect A threonine at position 241 changed the binding preference of the coat protein toward genomic RNA.
- Intervention effect A threonine at position 241 inhibited cell-to-cell movement of Bamboo mosaic virus.
- Mechanism Phosphorylation of the coat protein by NbCK2α regulates cell-to-cell movement of Bamboo mosaic virus by modulating RNA binding affinity.