Claim · model-written summary
All three PSV coat protein isolates yielded RT PCR amplicons of the expected 651 bp size.
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Verbatim
e0“Amplicons of the expected size (651 bp) were obtained from the coat protein region for all three PSV CP isolates”
e1“Electron microscopy of bean extracts”
Title
First report of Peanut stunt virus on beans in Bulgaria
Pasev, G.; Radeva-Ivanova, V.; Y. Manoussopoulos; et al. Zenodo (CERN European Organization for Nuclear Research) 2018. doi:10.5281/zenodo.3693664
Other claims from this paper (17)
- Observation Disease incidence in symptomatic bean fields ranged from 10 to 50%.
- Observation Symptomatic bean plants had green or yellow mosaic, blisters, and leaf distortion.
- Observation Severe stunting accompanied prevalent disease in some bean locations.
- Observation BCMV, CMV, BYMV and ClYVV occurred in the majority of symptomatic bean samples.
- Observation Three symptomatic bean samples tested positive for Peanut stunt virus.
- Association Peanut stunt virus positive bean samples came from fields where stunting was prevalent.
- Intervention effect Sap inoculation with PSV infected samples caused local chlorotic lesions in Chenopodium amaranticolor.
- Intervention effect Sap inoculation with PSV infected samples caused leaf distortion in Nicotiana benthamiana.
- Intervention effect Mechanical inoculation of PSV isolates caused systemic chlorotic lesions and rings in Pinto bean plants.
- Intervention effect Mechanical inoculation of PSV isolates caused local chlorotic lesions and later systemic chlorotic lesions in Chenopodium quinoa.
- Intervention effect Mechanical inoculation of PSV isolates caused local necrotic lesions in Chenopodium murale.
- Observation Electron microscopy of bean extracts revealed isometric particles about 28 nm in diameter.
- Method / resource Sequencing the bean associated amplicons confirmed PSV identification.
- Method / resource Sequences of the bean associated PSV isolates were deposited as MG256178 through MG256180.
- Observation Phylogenetic analysis placed all three Bulgarian PSV isolates in Western subgroup II.
- Observation The authors identify this as the first report of PSV in Bulgaria and the second report of the Western strain in Europe.
- Recommendation The authors propose monitoring natural hosts to clarify PSV epidemiology in Eastern Europe.