Claim · model-written summary
The radiolabeling method had a lack of sensitivity and a failure to distinguish attached bacteria compared with direct counting by phase-contrast microscopy.
- Taxon
- Trifolium repens
Trifolium repenst0 - Outcome
- lack of sensitivitye3
- Qualifier
- failure to distinguish between bacteria attached to mature root hairs, emerging root hairs, and undifferentiated epidermal cellse3
- Comparator
- direct counting by using phase-contrast microscopye4
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Association of Rhizobium Strains with Roots of Trifolium repens”
e3“drawbacks of this method included its lack of sensitivity and its failure to distinguish between bacteria attached to mature root hairs, emerging root hairs, and undifferentiated epidermal cells”
e4“indirect counting after radiolabeling the bacteria and direct counting by using phase-contrast microscopy”
Title
Association of Rhizobium Strains with Roots of Trifolium repens
Jane Badenoch-Jones; David J. Flanders; Barry G. Rolfe Applied and Environmental Microbiology 1985. doi:10.1128/aem.49.6.1511-1520.1985
Other claims from this paper (8)
- Observation Bacteria associated with individual clover root hairs varied largely in number.
- Method / resource Reproducibility of results from the radiolabeling method was satisfactory.
- Observation The method failed to distinguish between individual bacteria and aggregates.
- Observation Binding of R. trifolii to clover roots showed no indication of specificity.
- Intervention effect 2-Deoxy-d-glucose did not have a major inhibitory effect on rhizobial attachment to the clover root.
- Mechanism Lectin cross-bridging is not an obligatory step in the initiation of infection, though it may occur under some conditions.
- Association The presence or absence of the symbiotic plasmid was not correlated with bacterial adherence to the host plant root.
- Mechanism Binding of rhizobia to the legume root is not the basis of host specificity.