Claim · model-written summary
Labeled pyrroline-5-carboxylate appeared in bacteroids but not host cells after two hours.
- Taxon
- Soybean
Glycine maxt0 - Outcome
- [(3)H] Pyrroline-5-carboxylatee0
- Value
- 2-houre0
- Qualifier
- bacteroids, but not host cellse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“[(3)H] Pyrroline-5-carboxylate was found in bacteroids, but not host cells, after a 2-hour incubation in [5-(3)H]l-proline”
t0“Soybean”
Title
Yuxian Zhu; Georgia Shearer; Daniel H. Kohl PLANT PHYSIOLOGY 1992. doi:10.1104/pp.98.3.1020
Other claims from this paper (14)
- Intervention effect Root-supplied proline stimulated acetylene reducing activity as much as succinate.
- Intervention effect Proline feeding increased bacteroid proline dehydrogenase activity.
- Association Bacteroid proline dehydrogenase activity correlated strongly with acetylene reducing activity.
- Intervention effect Proline content in host cytoplasm rose about threefold after 24 hours.
- Intervention effect Bacteroid proline content rose about eightfold after 24 hours.
- Observation Bacteroid proline concentration was calculated to be at least 3.5 millimolar.
- Observation Radiolabeled proline and its products accumulated in host cells and bacteroids.
- Observation Tritium-labeled proline was found in host cells and bacteroids.
- Observation After 24 hours, labeled pyrroline-5-carboxylate occurred in host cells at about half its bacteroid quantity.
- Mechanism Proline crossed both plant and bacterial membranes under the experiment conditions.
- Mechanism Proline may supply energy for bacteroid functioning.
- Intervention effect Proline did not rescue acetylene reducing activity in stemgirdled plants.
- Intervention effect Succinate rescued acetylene reducing activity in stemgirdled plants.
- Mechanism Succinate, but not proline, may reach nodules by a nonphloem route.