Claim · model-written summary
Density gradient centrifugation produced cytoplast and miniprotoplast preparations from winged bean.
- Taxon
- winged bean
Psophocarpus tetragonolobuse0 - Outcome
- cytoplasts and miniprotoplastse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Protoplasts derived from suspension cultures of the winged bean and a crown gall lineof Parthenocissus tricuspidata were enucleated by centrifugation on iso-osmotic discontinuous Percoli gradients and on discontinuous sucrose-mannitol density gradients. Enucleation was achieved according to the buoyant density of the protoplasts and preparations of both cytoplasts and miniprotoplasts were recovered”
Title
H. Fakhrai; H. Haq; P. K. Evans Biologia Plantarum 1988. doi:10.1007/bf02890507
Other claims from this paper (4)
- Method / resource Density gradient centrifugation produced cytoplast and miniprotoplast preparations from Parthenocissus.
- Method / resource Percoll gradients gave more satisfactory enucleation results than sucrose-mannitol gradients.
- Intervention effect Increasing centrifugal force from 20 kg to 60 kg increased enucleation.
- Observation About 85% of cytoplasts retained an intact plasma membrane.