Abstract
Protoplasts derived from suspension cultures of the winged bean and a crown gall lineof Parthenocissus tricuspidata were enucleated by centrifugation on iso-osmotic discontinuous Percoli gradients and on discontinuous sucrose-mannitol density gradients. Enucleation was achieved according to the buoyant density of the protoplasts and preparations of both cytoplasts and miniprotoplasts were recovered. The Percoll gradients gave more satisfactory results than the sucrose-mannitol gradients. Enucleation was increased by raising the centrifugal force from 20 kg to 60 kg. The presence of nuclei was determined by the DNA specific probe 4′6-diamidino-2-phenylindole (DAPI). Membrane integrity, as measured by staining with fluorescein diacetate, showed that some 85 % of the cytoplasts were bounded by an intact plasma membrane.
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Claims 5
Claim · model-written summary
Density gradient centrifugation produced cytoplast and miniprotoplast preparations from winged bean.
- Taxon
- winged bean
Psophocarpus tetragonolobuse0 - Outcome
- cytoplasts and miniprotoplastse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts derived from suspension cultures of the winged bean and a crown gall lineof Parthenocissus tricuspidata were enucleated by centrifugation on iso-osmotic discontinuous Percoli gradients and on discontinuous sucrose-mannitol density gradients. Enucleation was achieved according to the buoyant density of the protoplasts and preparations of both cytoplasts and miniprotoplasts were recovered”
Claim · model-written summary
Density gradient centrifugation produced cytoplast and miniprotoplast preparations from Parthenocissus.
- Taxon
- Parthenocissus tricuspidata
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Parthenocissus tricuspidatae0 - Outcome
- cytoplasts and miniprotoplastse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Protoplasts derived from suspension cultures of the winged bean and a crown gall lineof Parthenocissus tricuspidata were enucleated by centrifugation on iso-osmotic discontinuous Percoli gradients and on discontinuous sucrose-mannitol density gradients. Enucleation was achieved according to the buoyant density of the protoplasts and preparations of both cytoplasts and miniprotoplasts were recovered”
Claim · model-written summary
Percoll gradients gave more satisfactory enucleation results than sucrose-mannitol gradients.
- Taxon
- winged beanPsophocarpus tetragonolobust0
- Outcome
- more satisfactory resultse0
- Direction
- more satisfactorye0
- Comparator
- sucrose-mannitol gradientse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Percoll gradients gave more satisfactory results than the sucrose-mannitol gradients”
t0“winged bean”
Claim · model-written summary
Increasing centrifugal force from 20 kg to 60 kg increased enucleation.
- Taxon
- winged beanPsophocarpus tetragonolobust0
- Treatment
- raising the centrifugal force from 20 kg to 60 kge0
- Outcome
- Enucleatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enucleation was increased by raising the centrifugal force from 20 kg to 60 kg”
t0“winged bean”
Claim · model-written summary
About 85% of cytoplasts retained an intact plasma membrane.
- Taxon
- winged beanPsophocarpus tetragonolobust0
- Outcome
- intact plasma membranee0
- Value
- 85 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some 85 % of the cytoplasts were bounded by an intact plasma membrane”
t0“winged bean”