Claim · model-written summary
A transgenic Arabidopsis thaliana plant line expressing the switchable fluorescent protein rsEGFP2 was generated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- transgenic Arabidopsis thaliana plant linee2
- Gene
- rsEGFP2e2
- Outcome
- expressing the reversibly switchable fluorescent protein rsEGFP2e2
Not reportedCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis thaliana”
e2“We generated a transgenic Arabidopsis thaliana plant line expressing the reversibly switchable fluorescent protein rsEGFP2”
Title
Live‐cell RESOLFT nanoscopy of transgenic Arabidopsis thaliana
Sebastian Schnorrenberg; Hassan Ghareeb; Lars Frahm; et al. Plant Direct 2020. doi:10.1002/pld3.261
Other claims from this paper (5)
- Method / resource Live-cell RESOLFT nanoscopy was established on living green plant tissue.
- Method / resource Live-cell RESOLFT nanoscopy uses comparatively low light doses and intensities to overcome the diffraction barrier.
- Method / resource Extended imaging of cortical microtubules in Arabidopsis leaf discs was demonstrated with RESOLFT nanoscopy.
- Method / resource Combining RESOLFT nanoscopy with fluorescence lifetime gating allowed live-cell images close to chloroplasts despite their very strong autofluorescence.
- Method / resource Subdiffraction-resolution imaging is feasible in transgenic plant material with minimal sample preparation.