Claim · model-written summary
Live-cell RESOLFT nanoscopy was established on living green plant tissue.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Outcome
- RESOLFT nanoscopye0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis thaliana”
e0“we established RESOLFT nanoscopy on living green plant tissue”
Title
Live‐cell RESOLFT nanoscopy of transgenic Arabidopsis thaliana
Sebastian Schnorrenberg; Hassan Ghareeb; Lars Frahm; et al. Plant Direct 2020. doi:10.1002/pld3.261
Other claims from this paper (5)
- Method / resource Live-cell RESOLFT nanoscopy uses comparatively low light doses and intensities to overcome the diffraction barrier.
- Method / resource A transgenic Arabidopsis thaliana plant line expressing the switchable fluorescent protein rsEGFP2 was generated.
- Method / resource Extended imaging of cortical microtubules in Arabidopsis leaf discs was demonstrated with RESOLFT nanoscopy.
- Method / resource Combining RESOLFT nanoscopy with fluorescence lifetime gating allowed live-cell images close to chloroplasts despite their very strong autofluorescence.
- Method / resource Subdiffraction-resolution imaging is feasible in transgenic plant material with minimal sample preparation.