Claim · model-written summary
The specified sucrose preculture and temperature sequence yielded 60% maximum survival.
- Taxon
- black irist0
- Treatment
- Preculturing embryos on medium containing 0.75 M sucrose for 3 d at 22 degree C, then at 30 degree C for 1 de0
- Outcome
- survivale0
- Direction
- maximume0
- Value
- 60%e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Verbatim
e0“Preculturing embryos on medium containing 0.75 M sucrose for 3 d at 22 degree C, then at 30 degree C for 1 d ensured maximum survival (60%).”
t0“black iris (Iris nigricans)”
Title
Cryopreservation of black iris (Iris nigricans) somatic embryos by encapsulation-dehydration.
R A Shibli PubMed
Other claims from this paper (7)
- Method / resource Black iris somatic embryos were cryopreserved by encapsulation-dehydration.
- Association Embryos 2-4 mm long had the highest survival after cryopreservation.
- Observation Viable embryos were brown after cryopreservation and early recovery; dead embryos were light brown or creamy.
- Observation First regrowth appeared after 15 d.
- Observation Living embryo regrowth reached 90% after 35 d.
- Observation Only 10% of embryos showed secondary embryogenesis.
- Intervention effect Direct in vivo sowing of cryopreserved embryos did not achieve germination.