Abstract
Somatic embryos of black iris (Iris nigricans) were cryopreserved using encapsulation-dehydration. Embryos size of 2-4 mm gave the highest survival after cryopreservation. Preculturing embryos on medium containing 0.75 M sucrose for 3 d at 22 degree C, then at 30 degree C for 1 d ensured maximum survival (60%). Viable embryos were brown in color after cryopreservation and during early recovery while dead ones where light brown or creamy. The first sign of regrowth was noted after 15 d. The final regrowth percentage of living embryo was 90% after 35 d. Only 10% of the embryos in all treatments showed secondary embryogenesis. Direct sowing in vivo of cryopreserved embryos was not successful in achieving germination.
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Claims 8
Claim · model-written summary
Black iris somatic embryos were cryopreserved by encapsulation-dehydration.
Not reportedComparatorValue
Verbatim
e0“Somatic embryos of black iris (Iris nigricans) were cryopreserved using encapsulation-dehydration.”
Claim · model-written summary
Embryos 2-4 mm long had the highest survival after cryopreservation.
Verbatim
e0“Embryos size of 2-4 mm gave the highest survival after cryopreservation.”
t0“black iris (Iris nigricans)”
Claim · model-written summary
The specified sucrose preculture and temperature sequence yielded 60% maximum survival.
- Taxon
- black irist0
- Treatment
- Preculturing embryos on medium containing 0.75 M sucrose for 3 d at 22 degree C, then at 30 degree C for 1 de0
- Outcome
- survivale0
- Direction
- maximume0
- Value
- 60%e0
Not reportedComparator
Verbatim
e0“Preculturing embryos on medium containing 0.75 M sucrose for 3 d at 22 degree C, then at 30 degree C for 1 d ensured maximum survival (60%).”
t0“black iris (Iris nigricans)”
Claim · model-written summary
Viable embryos were brown after cryopreservation and early recovery; dead embryos were light brown or creamy.
Not reportedDirection
Verbatim
e0“Viable embryos were brown in color after cryopreservation and during early recovery while dead ones where light brown or creamy.”
t0“black iris (Iris nigricans)”
Claim · model-written summary
First regrowth appeared after 15 d.
Not reportedDirection
Verbatim
e0“The first sign of regrowth was noted after 15 d.”
t0“black iris (Iris nigricans)”
Claim · model-written summary
Living embryo regrowth reached 90% after 35 d.
Not reportedDirection
Verbatim
e0“The final regrowth percentage of living embryo was 90% after 35 d.”
t0“black iris (Iris nigricans)”
Claim · model-written summary
Only 10% of embryos showed secondary embryogenesis.
Not reportedDirection
Verbatim
e0“Only 10% of the embryos in all treatments showed secondary embryogenesis.”
t0“black iris (Iris nigricans)”
Claim · model-written summary
Direct in vivo sowing of cryopreserved embryos did not achieve germination.
Not reportedComparatorDirection
Verbatim
e0“Direct sowing in vivo of cryopreserved embryos was not successful in achieving germination.”
t0“black iris (Iris nigricans)”