Method / resourcemodel-written summary

Lysis of oocysts and cysts was rapidly achieved within 3 min on lettuce, a prerequisite for sensitive detection.

A claim from Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol · 2025

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Lysis of oocysts and cysts was rapidly achieved within 3 min on lettuce, a prerequisite for sensitive detection.

Taxon
LettuceLactuca sativae1
Outcome
lysis of oocysts and cystse6
Value
within 3 mine6

Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e1

“Lettuce (25 g) was spiked with varying numbers of Cryptosporidium parvum oocysts”

✓ located verbatim in the source · abstract, characters 575–655
e6

“The efficient lysis of oocysts and cysts was a prerequisite for the sensitive detection of parasite DNA and was rapidly achieved within 3 min”

✓ located verbatim in the source · abstract, characters 1321–1462

Title

Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol

Sohail Naushad; Ruimin Gao; Marc-Olivier Duceppe; et al. Frontiers in Microbiology 2025. doi:10.3389/fmicb.2025.1566579

Other claims from this paper (4)

Record rev-950292dce56f7cc0bc398bfe0ac56d40961f0c89ab13990da914ea1211329fee · schema claims-v0.9 · position 5 in the model’s answer