Claim · model-written summary
Lysis of oocysts and cysts was rapidly achieved within 3 min on lettuce, a prerequisite for sensitive detection.
- Taxon
- Lettuce
Lactuca sativae1 - Outcome
- lysis of oocysts and cystse6
- Value
- within 3 mine6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“Lettuce (25 g) was spiked with varying numbers of Cryptosporidium parvum oocysts”
e6“The efficient lysis of oocysts and cysts was a prerequisite for the sensitive detection of parasite DNA and was rapidly achieved within 3 min”
Title
Sohail Naushad; Ruimin Gao; Marc-Olivier Duceppe; et al. Frontiers in Microbiology 2025. doi:10.3389/fmicb.2025.1566579
Other claims from this paper (4)
- Method / resource The mNGS assay identified as few as 100 oocysts of C. parvum in 25 g of fresh lettuce.
- Method / resource Ion S5 sequencing gave results similar to Nanopore sequencing for the mNGS assay on lettuce.
- Observation The assay simultaneously detected C. parvum and G. duodenalis on lettuce, among other protozoa.
- Method / resource Amplification gave 0.16–8.25 μg of DNA from lettuce washes, sufficient to perform mNGS.