Claim · model-written summary
6K2 separation is important for viral replication rate and movement.
Not reportedTaxonMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“proteolytic separation of the 6K2 protein from the adjacent proteins by NIaPro is important for the rate of virus replication and movement”
Title
Andres Merits; Minna-Liisa Rajamäki; Päivi Lindholm; et al. Journal of General Virology 2002. doi:10.1099/0022-1317-83-5-1211
Other claims from this paper (14)
- Observation P3/6K1, CI-6K2, and VPg/NIaPro sites processed slowly.
- Observation Other cleavage sites processed at a high rate.
- Observation CI-6K2 polyprotein occurred in infected plant cells.
- Observation Most CI-6K2 was membrane-associated in both cell types, unlike processed CI.
- Intervention effect Deleting 6K1 prevented proper P3 separation from CI.
- Intervention effect Deleting 6K1 did not affect processing of VPg, NIaPro, NIb, or CP.
- Intervention effect Removing 6K2 sequence had no detectable processing effect.
- Intervention effect Deleting 6K1 rendered PVA non-infectious.
- Intervention effect Deleting 6K2 rendered PVA non-infectious.
- Intervention effect 6K2/VPg mutations reduced viral infectivity in plants.
- Intervention effect 6K2/VPg mutations had a smaller detectable processing effect in the baculovirus system.
- Mechanism NIaPro preferentially catalyzes cis cleavage.
- Mechanism 6K1/CI and NIb/CP sites can be processed in trans.
- Mechanism Both 6K peptides are indispensable for virus replication.