Claim · model-written summary
At infinitely high inhibitor concentration, glycylsarcosine completely inhibited Gly-Gly uptake.
- Taxon
- barley
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Hordeum vulgaree2 - Treatment
- glycylsarcosinee1
- Outcome
- uptakee0
- Direction
- completely inhibitede0
- Qualifier
- infinitely high inhibitor concentratione0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“completely inhibited the uptake at infinitely high inhibitor concentration”
e1“The poorly hydrolyzed "model" dipeptide glycylsarcosine”
e2“germinating barley grains”
Title
Uptake of Glycylglycine by the Scutellum of Germinating Barley Grain
Tuomas Sopanen; David Burston; Elizabeth Taylor; et al. PLANT PHYSIOLOGY 1978. doi:10.1104/pp.61.4.630
Other claims from this paper (19)
- Observation Barley scutella rapidly took up labeled glycylglycine.
- Observation Gly-Gly uptake had a pH optimum near 4.5.
- Observation Gly-Gly uptake followed Michaelis-Menten kinetics with apparent Km of 2.3 mm.
- Observation Gly-Gly uptake had Vmax of 41 mumole gram(-1) hour(-1).
- Intervention effect Dinitrophenol strongly inhibited Gly-Gly uptake.
- Intervention effect Cyanide strongly inhibited Gly-Gly uptake.
- Intervention effect Lack of oxygen strongly inhibited Gly-Gly uptake.
- Observation No intact Gly-Gly was detectable in scutella after incubation.
- Intervention effect Free glycine increased in scutella after incubation with Gly-Gly.
- Intervention effect Glycylsarcosine competitively inhibited Gly-Gly uptake.
- Mechanism Gly-Gly was inferred to enter as an intact dipeptide and then be hydrolyzed to glycine.
- Intervention effect Glycine did not affect Gly-Gly uptake.
- Intervention effect Leucine did not affect Gly-Gly uptake.
- Intervention effect All ten tested dipeptides strongly inhibited Gly-Gly uptake.
- Observation Barley scutella took up Ala-Gly.
- Observation Barley scutella took up Pro-Gly.
- Observation Barley scutella took up Gly-Pro.
- Mechanism Dipeptide uptake appeared nonspecific to amino acid side chains.
- Mechanism High dipeptide uptake rates suggest a role in reserve protein mobilization.