Claim · model-written summary
Expression of OsNAR2.1 and OsNRAMP3 was specifically induced under low-nitrate conditions (0.2 mM NO3-).
- Taxon
- rice
Oryza sativat0 - Treatment
- low-nitrate conditions (0.2 mM NO3−)e0
- Outcome
- expressione0
- Direction
- inducede0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Their expression followed rhythmic patterns (Figure S3E), was downregulated under Fe2+ deficiency (Figures S3F, G), and was specifically induced under low-nitrate conditions (0.2 mM NO3−)”
Title
A partner protein helps OsNRAMP3 win a novel function to transport Fe 2+ in rice
Jinxia Xiang; Jingguang Chen; Biqi Lei; et al. Journal of Integrative Plant Biology 2025. doi:10.1111/jipb.70105
Other claims from this paper (19)
- Intervention effect Expression of pOsNAR2.1:OsNAR2.1 in rice significantly increased Fe2+ and Mn2+ concentrations and contents under nitrate conditions.
- Observation osnar2.1 mutants showed reduced root and shoot Fe2+ concentrations at 0.2 mM NO3-.
- Observation osnar2.1 mutants did not show reduced root and shoot Fe2+ concentrations at 2.5 mM NH4+.
- Observation The interaction of OsNAR2.1 with OsNRAMP3, suggested by a pull-down assay, was confirmed by Y2H, BiFC, LCI and Co-IP assays.
- Observation In the Fe-uptake-deficient yeast mutant DEY1453, neither OsNRAMP3 nor OsNAR2.1 alone complemented Fe2+ transport, but their co-expression restored it.
- Intervention effect Co-expressing OsNAR2.1 and OsNRAMP3 in Xenopus laevis oocytes significantly enhanced Fe2+ accumulation in the presence of 1 mM Fe2+ plus 1 mM NO3-.
- Intervention effect Co-expressing OsNAR2.1 and OsNRAMP3 in Xenopus laevis oocytes did not enhance Fe2+ accumulation with Fe2+ alone.
- Observation OsNAR2.1 and OsNRAMP3 were predominantly expressed in Node I of rice, with the highest levels in Nipponbare and Dongjin cultivars.
- Intervention effect Expression of OsNAR2.1 and OsNRAMP3 was downregulated under Fe2+ deficiency.
- Observation Under 0.2 mM NO3-, all osnramp3 mutants displayed reduced biomass and total nitrogen accumulation.
- Observation In osnramp3 mutants after nitrogen starvation, 15N uptake significantly decreased under low nitrate, with higher 15N in roots, lower in shoots and significantly lower shoot-to-root 15N ratios.
- Observation Under Fe2+ deficiency, the root Fe2+ concentration of osnramp3 mutants significantly decreased.
- Mechanism OsNAR2.1 confers Fe2+ transport function on OsNRAMP3 in rice.
- Association OsNRAMP3 expression was downregulated in osnar2.1 mutants and upregulated in OsNAR2.1-overexpression lines, whereas OsNAR2.1 expression was unchanged in osnramp3 mutants.
- Observation The OsNAR2.1-overexpression line OE1 increased grain yield and seed-setting rate.
- Observation The hybrid OE1/NG3081 showed no improvement in grain yield and seed-setting rate over NG3081.
- Observation The hybrid OE1/NG4081 partially rescued the grain yield and seed-setting phenotype of NG4081.
- Observation OsNRAMP3 localization shifts from the plasma membrane in wild type to the endoplasmic reticulum in osnar2.1 mutants.
- Recommendation The OsNAR2.1-OsNRAMP3 module is proposed as a promising Fe-biofortification strategy to develop high-yield, high-Fe rice.