Claim · model-written summary
TaIAR3 has low specificity for the ester conjugates IAA-Glc and IAA-myoinositol.
- Taxon
- wheat
Triticum aestivume1 - Gene
- TaIAR3e1
- Compound
- IAA-Glc and IAA-myoinositole2
- Outcome
- low specificitye2
Not reportedMaterialOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An ortholog for the Arabidopsis IAR3 auxin amidohydrolase gene has been isolated from wheat (TaIAR3)”
e2“has low specificity for the ester conjugates IAA-Glc and IAA-myoinositol”
Title
James J. Campanella; Adebanke F. Olajide; Volker Magnus; et al. PLANT PHYSIOLOGY 2004. doi:10.1104/pp.104.043398
Other claims from this paper (11)
- Observation An ortholog of the Arabidopsis IAR3 gene was isolated from wheat as TaIAR3.
- Observation TaIAR3 hydrolyzes negligible levels of IAA-Ala and no other IAA amino acid conjugates tested, unlike IAR3.
- Observation TaIAR3 has high specificity for the conjugates of IBA with Ala and Gly.
- Observation TaIAR3 has high specificity for the conjugate IPA-Ala so far tested.
- Observation TaIAR3 did not convert the methyl esters of the IBA conjugates with Ala and Gly.
- Observation IBA and IBA conjugates were detected in wheat seedlings by gas chromatography-mass spectrometry.
- Mechanism The conjugate of IBA with Ala may serve as a natural substrate for the enzyme (hypothesis).
- Observation Endogenous IPA and IPA conjugates were not detected in wheat seedlings.
- Observation Crude protein extracts of wheat seedlings possess auxin amidohydrolase activity.
- Association TaIAR3 transcript is initially expressed at day 1 after germination and its expression decreases through day 20.
- Observation TaIAR3 expression levels are similar throughout all wheat tissues examined.