Claim · model-written summary
Phenylalanine at 5 mM gave 55.3% of explants producing somatic embryos.
- Treatment
- supplementation of 5 mM phenylalaninee0
- Outcome
- number of explants showing somatic embryo productione0
- Direction
- highere0
- Value
- 55.3 %e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher number of explants showing somatic embryo production (55.3 %) and higher number of somatic embryos per explant (15.5 per explant) were recorded with the supplementation of 5 mM phenylalanine”
t0“grapevine 'Crimson Seedless'”
Title
Use of amino acids for a highly efficient somatic embryogenesis in grapevine 'Crimson Seedless'
Nookaraju, A.; Agrawal, D. C. Federal Research Centre for Cultivated Plants (Julius Kühn-Institut) 2015. doi:10.5073/vitis.2013.52.137-140
Other claims from this paper (7)
- Intervention effect BA at 4.5 μM induced a higher embryogenic response and more somatic embryos per explant.
- Intervention effect Adding 5 μM NOA increased the embryogenic response on half strength MS medium with 4.5 μM BA.
- Intervention effect Amino acid supplementation significantly improved leaf embryogenic response.
- Intervention effect Phenylalanine at 5 mM gave 15.5 somatic embryos per explant.
- Observation Primary somatic embryos underwent repetitive embryogenesis on half strength MS without growth regulators.
- Observation Hardened embryo derived plantlets established in a greenhouse with 90% survival.
- Method / resource The somatic embryogenesis system was used for Agrobacterium-mediated transformation studies.