Claim · model-written summary
R. trifolii capsular material bound specifically to clover root hairs.
- Organ / stage
- root hairse0
- Interactor
- R. trifolii
Rhizobium leguminosarume0symbiont - Outcome
- bound specifically to root hairse0
TrifoliumNot reportedMaterialGeneCompoundSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescein isothiocyanate-labeled capsular material from R. trifolii bound specifically to root hairs of clover but not alfalfa.”
Title
Receptor Site on Clover and Alfalfa Roots for Rhizobium
Frank B. Dazzo; Winston J. Brill Applied and Environmental Microbiology 1977. doi:10.1128/aem.33.1.132-136.1977
Other claims from this paper (12)
- Observation R. trifolii capsular material did not bind to alfalfa root hairs.
- Observation Binding was most intense at clover root hair tips.
- Intervention effect 2-deoxyglucose treatment prevented R. trifolii capsular material binding to clover roots.
- Intervention effect 2-dG enhanced clover root protein elution.
- Observation Eluted clover root protein bound and specifically agglutinated R. trifolii.
- Observation Eluted clover root protein did not agglutinate R. meliloti.
- Observation Eluted clover root protein did not agglutinate R. japonicum.
- Method / resource The mild elution procedure left roots intact.
- Intervention effect 2-dG specifically inhibited R. trifolii agglutination.
- Intervention effect 2-dG specifically inhibited passive hemagglutination of rabbit erythrocytes bearing R. trifolii capsular material.
- Mechanism The authors propose clover root proteins cross-link complementary root-hair and rhizobial polysaccharides through 2-dG-sensitive binding sites.
- Observation Alfalfa root hairs specifically bound a surface polysaccharide from R. meliloti.