Abstract
Sites on white clover and alfalfa roots that bind Rhizobium trifolii and R. meliloti capsular polysaccharides, respectively, were examined by fluorescence microscopy. Fluorescein isothiocyanate-labeled capsular material from R. trifolii bound specifically to root hairs of clover but not alfalfa. Binding was most intense at the root hair tips. Treatment of clover roots with 2-deoxyglucose (2-dG) prevented binding of R. trifolii capsular material to the roots. The sugar 2-dG enhanced the elution of clover root protein, which could bind to and specifically agglutinate R. trifolii but not R. meliloti or R. japonicum. The mild elution procedure left the roots intact. Agglutination of R. trifolii and passive hemagglutination of rabbit erythrocytes coated with the capsular material of R. trifolii were specifically inhibited by 2-dG. These results suggest that clover roots contain proteins that cross-link complementary polysaccharides on the surface of clover root hairs and infective R. trifolii through 2-dG-sensitive binding sites. Alfalfa root hairs were shown to specifically bind to a surface polysaccharide from R. meliloti.
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Claims 13
Claim · model-written summary
R. trifolii capsular material bound specifically to clover root hairs.
- Organ / stage
- root hairse0
- Interactor
- R. trifolii
Rhizobium leguminosarume0symbiont - Outcome
- bound specifically to root hairse0
TrifoliumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescein isothiocyanate-labeled capsular material from R. trifolii bound specifically to root hairs of clover but not alfalfa.”
Claim · model-written summary
R. trifolii capsular material did not bind to alfalfa root hairs.
- Taxon
- alfalfa
Medicago sativae0 - Interactor
- R. trifoliiRhizobium leguminosarume0symbiont
- Outcome
- but not alfalfae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescein isothiocyanate-labeled capsular material from R. trifolii bound specifically to root hairs of clover but not alfalfa.”
Claim · model-written summary
Binding was most intense at clover root hair tips.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Binding was most intense at the root hair tips.”
e1“root hairs of clover”
Claim · model-written summary
2-deoxyglucose treatment prevented R. trifolii capsular material binding to clover roots.
- Treatment
- 2-deoxyglucose (2-dG)e0
- Interactor
- R. trifoliiRhizobium leguminosarume0symbiont
- Outcome
- binding of R. trifolii capsular materiale0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of clover roots with 2-deoxyglucose (2-dG) prevented binding of R. trifolii capsular material to the roots.”
Claim · model-written summary
2-dG enhanced clover root protein elution.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sugar 2-dG enhanced the elution of clover root protein, which could bind to and specifically agglutinate R. trifolii but not R. meliloti or R. japonicum.”
Claim · model-written summary
Eluted clover root protein bound and specifically agglutinated R. trifolii.
- Interactor
- R. trifoliiRhizobium leguminosarume0symbiont
- Outcome
- bind to and specifically agglutinate R. trifoliie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clover root protein, which could bind to and specifically agglutinate R. trifolii but not R. meliloti or R. japonicum”
Claim · model-written summary
Eluted clover root protein did not agglutinate R. meliloti.
- Interactor
- R. meliloti
Sinorhizobium melilotie0symbiont - Outcome
- but not R. melilotie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clover root protein, which could bind to and specifically agglutinate R. trifolii but not R. meliloti or R. japonicum”
Claim · model-written summary
Eluted clover root protein did not agglutinate R. japonicum.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clover root protein, which could bind to and specifically agglutinate R. trifolii but not R. meliloti or R. japonicum”
Claim · model-written summary
The mild elution procedure left roots intact.
- Outcome
- roots intacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mild elution procedure left the roots intact.”
e1“clover root protein”
Claim · model-written summary
2-dG specifically inhibited R. trifolii agglutination.
- Taxon
- R. trifoliiRhizobium leguminosarume0
- Treatment
- 2-dGe0
- Outcome
- Agglutination of R. trifoliie0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agglutination of R. trifolii and passive hemagglutination of rabbit erythrocytes coated with the capsular material of R. trifolii were specifically inhibited by 2-dG.”
Claim · model-written summary
2-dG specifically inhibited passive hemagglutination of rabbit erythrocytes bearing R. trifolii capsular material.
- Taxon
- rabbit
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Oryctolagus cuniculuse0 - Treatment
- 2-dGe0
- Outcome
- passive hemagglutination of rabbit erythrocytese0
- Direction
- inhibitede0
- Qualifier
- coated with the capsular material of R. trifoliie0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Agglutination of R. trifolii and passive hemagglutination of rabbit erythrocytes coated with the capsular material of R. trifolii were specifically inhibited by 2-dG.”
Claim · model-written summary
The authors propose clover root proteins cross-link complementary root-hair and rhizobial polysaccharides through 2-dG-sensitive binding sites.
- Interactor
- R. trifoliiRhizobium leguminosarume0symbiont
- Outcome
- cross-link complementary polysaccharidese0
- Qualifier
- through 2-dG-sensitive binding sitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that clover roots contain proteins that cross-link complementary polysaccharides on the surface of clover root hairs and infective R. trifolii through 2-dG-sensitive binding sites.”
Claim · model-written summary
Alfalfa root hairs specifically bound a surface polysaccharide from R. meliloti.
- Taxon
- AlfalfaMedicago sativae0
- Organ / stage
- root hairse0
- Interactor
- R. melilotiSinorhizobium melilotie0symbiont
- Outcome
- specifically bind to a surface polysaccharidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa root hairs were shown to specifically bind to a surface polysaccharide from R. meliloti.”