Claim · model-written summary
GhMYC2-D09 activated GhCAD1-A expression in dual-luciferase assays.
- Taxon
- cotton
Gossypium hirsutumt0 - Gene
- GhMYC2-D09e0
- Outcome
- expression of GhCAD1-Ae0
- Qualifier
- activatee0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMYC2-D09 can activate the expression of GhCAD1-A and GhCAD1-C”
t0“cottonseed”
Title
CRISPR/Cas9-mediated mutation of GhCAD decreases the gossypol content of cottonseed
Lili Zhou; Yali Wang; Jiamin Wang; et al. Journal of Biological Engineering 2025. doi:10.1186/s13036-025-00556-2
Other claims from this paper (14)
- Intervention effect Targeted GhCAD mutation reduced gossypol levels in cottonseed and leaves by about 64%.
- Intervention effect Editing only GhCAD1-A reduced seed gossypol content by about 46%.
- Intervention effect Editing only GhCAD1-A caused no major change in leaf gossypol content.
- Intervention effect Transgenic and control cotton seeds had similar protein profiles.
- Intervention effect Transgenic and control cotton seeds had similar fatty acid profiles.
- Observation Jasmonic acid signal transduction was significantly enriched among the differentially expressed genes.
- Observation GhMYC2-D09 expression was down-regulated.
- Intervention effect Silencing GhMYC2-D09 decreased expression of gossypol biosynthesis genes.
- Intervention effect Silencing GhMYC2-D09 restricted gossypol accumulation in cotton leaves.
- Intervention effect Overexpressing GhMYC2-D09 in hairy roots had the opposite effect on gossypol accumulation.
- Mechanism GhMYC2-D09 activated GhCAD1-C expression in dual-luciferase assays.
- Observation GhMYC2-D09 did not bind directly to GhCAD promoters in Y1H assays.
- Method / resource The study developed genetic resources for low-gossypol cotton materials.
- Mechanism GhMYC2-D09 was characterized as a transcription factor that increases gossypol biosynthesis.