Claim · model-written summary
Phylogenetic analysis placed the ITS sequences of the isolates in the same clade as the AF284389 isolate.
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionValueQualifier
Verbatim
t0“cassava brown leaf spot in China”
e1“phylogenetic analysis showed that the sequences originating from our strains clustered in the same clade as the AF284389 isolate”
Title
Distribution and pathogen identification of cassava brown leaf spot in China
Y.L. Pei; T. Shi; C.P. Li; et al. Genetics and Molecular Research 2014. doi:10.4238/2014.april.30.7
Other claims from this paper (13)
- Observation Cassava plants were damaged by brown leaf spot to different degrees in most survey sites in China.
- Observation Seven strains were isolated from cassava leaf lesions.
- Observation Microconidia of the isolates formed by fragmentation on artificial medium plates.
- Intervention effect Stab inoculation of cassava leaves with conidia solution produced similar symptoms 14 days later.
- Observation Morphological characteristics of the specimens and conidia were similar to descriptions of Passalora henningsii infection.
- Observation The ITS rDNA regions of the isolates differed by three base pairs from that of the P. henningsii isolate AF284389.
- Observation The optimum temperature for mycelial growth of the isolates was 26° to 28°C.
- Observation Carrot agar medium was the optimum medium for mycelial growth of the isolates.
- Observation The optimum pH for mycelial growth of the isolates was 6.
- Observation Continuous dark was the optimum light condition for mycelial growth of the isolates.
- Observation Cassava leaf juice added to malt extract and cassava leaf juice added to potato dextrose agar were the best media for conidia production.
- Observation The optimal temperature for macroconidia germination was 26° to 28°C.
- Observation The lethal temperature for macroconidia germination was 60°C for 10 min.