Claim · model-written summary
The coat protein of 335 amino acids is the biggest SPFMV coat protein determined so far.
- Taxon
- SPFMVSweet potato feathery mottle viruse0
- Outcome
- coat protein sizee0
- Value
- 335 amino acidse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Verbatim
e0“The coat protein size of 335 amino acids is the biggest SPFMV so far determined”
Title
Survey and Characterization of Viruses in Sweetpotato from Zimbabwe
Farayi Chavi; A. Ian Robertson; Benedictus J. M. Verduin Plant Disease 1997. doi:10.1094/pdis.1997.81.10.1115
Other claims from this paper (9)
- Observation Systemic vein clearing appeared in N. benthamiana inoculated with buffered sap from nine sweetpotato clones.
- Observation Purification of the vein clearing agent from a sweetpotato clone gave yields of 2 to 17 mg/kg.
- Observation Electron microscopy showed flexuous filamentous particles with a modal length of 830 nm.
- Observation SDS-PAGE of purified virus showed a major protein band of 40 kDa, assumed to be the coat protein.
- Observation Minor protein bands of 27, 37 and 46 kDa were also observed.
- Observation The viral protein degraded on storage at 4 C to a 27 kDa band.
- Observation Labeling density of the purified virus was higher with SPFMV-RC antiserum than with SPCSV antiserum; the other antisera did not react.
- Observation RT-PCR with degenerate potyvirus primers and an oligo dT primer gave PCR products of correct size.
- Observation Compared with other SPFMV strains, the sequence was strongly similar except for a 22 amino acid insertion at the N-terminus of the coat protein.