Claim · model-written summary
RT-PCR with degenerate potyvirus primers and an oligo dT primer gave PCR products of correct size.
- Outcome
- PCR products of correct sizee0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“PCR products of correct size were obtained”
Title
Survey and Characterization of Viruses in Sweetpotato from Zimbabwe
Farayi Chavi; A. Ian Robertson; Benedictus J. M. Verduin Plant Disease 1997. doi:10.1094/pdis.1997.81.10.1115
Other claims from this paper (9)
- Observation Systemic vein clearing appeared in N. benthamiana inoculated with buffered sap from nine sweetpotato clones.
- Observation Purification of the vein clearing agent from a sweetpotato clone gave yields of 2 to 17 mg/kg.
- Observation Electron microscopy showed flexuous filamentous particles with a modal length of 830 nm.
- Observation SDS-PAGE of purified virus showed a major protein band of 40 kDa, assumed to be the coat protein.
- Observation Minor protein bands of 27, 37 and 46 kDa were also observed.
- Observation The viral protein degraded on storage at 4 C to a 27 kDa band.
- Observation Labeling density of the purified virus was higher with SPFMV-RC antiserum than with SPCSV antiserum; the other antisera did not react.
- Observation Compared with other SPFMV strains, the sequence was strongly similar except for a 22 amino acid insertion at the N-terminus of the coat protein.
- Observation The coat protein of 335 amino acids is the biggest SPFMV coat protein determined so far.