Claim · model-written summary
The duplex PCR assay detects the two pathogens simultaneously at a limit of about 0.05 ng/µL.
- Taxon
- cotton
Gossypium hirsutumt0 - Outcome
- simultaneously detect the two pathogense3
- Value
- 0.05 ng/µLe3
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“from cotton in Australia”
e3“a duplex PCR assay was developed to simultaneously detect the two pathogens at a limit of an approximately 0.05 ng/µL”
Title
Chi P. T. Nguyen; Andrew Chen; Duy P. Le Australasian Plant Pathology 2026. doi:10.1007/s13313-026-01096-z
Other claims from this paper (5)
- Method / resource The duplex PCR assay identifies B. rouxiae and B. basicola simultaneously without sequencing.
- Method / resource A new primer specifically amplifies a 159 bp amplicon of B. basicola.
- Method / resource The duplex PCR is sensitive enough to detect B. rouxiae from crude extracts prepared with a 5-min microwave-based DNA extraction.
- Method / resource Nested PCR combined with the duplex PCR successfully detects the BRR pathogen in 15–30 mg of diseased cotton tissue.
- Recommendation The duplex PCR is a valuable diagnostic tool for monitoring BRR pathogens in cotton in Australia.